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Literature summary for 3.1.1.4 extracted from
- Secretory phospholipase A2-alpha from Arabidopsis thaliana: functional parameters and substrate preference (2007), Chem. Phys. Lipids, 150, 156-166.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of AtsPLA2-alpha in Escherichia coli strain Bl21(DE3) in inclusion bodies | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Arabidopsis thaliana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
1,2-dioctanoyl-sn-glycero-3-phosphocholine | pH 8.5, 37°C, recombinant AtsPLA2-alpha | Arabidopsis thaliana | |
| 0.94 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | pH 8.5, 37°C, recombinant AtsPLA2-alpha, substrate below critical micelle concentration | Arabidopsis thaliana | |
| 3.85 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | pH 8.5, 37°C, recombinant AtsPLA2-alpha, substrate in presence of 2 M NaCl | Arabidopsis thaliana | |
| 3.92 | - |
1,2-diheptanoyl-sn-glycero-3-phosphocholine | pH 8.5, 37°C, recombinant AtsPLA2-alpha | Arabidopsis thaliana | |
| 13.85 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | pH 8.5, 37°C, recombinant AtsPLA2-alpha, substrate above critical micelle concentration | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Arabidopsis thaliana | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant AtsPLA2-alpha solubilized from Escherichia coli strain Bl21(DE3) inclusion bodies to homogeneity by gel filtration and anion exchange chromatography | Arabidopsis thaliana |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant AtsPLA2-alpha from Escherichia coli strain Bl21(DE3) inclusion bodies by 6 M guanidine hydrochloride followed by renaturation | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate application in micelles | Arabidopsis thaliana |
| 5.9 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-diheptanoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 9.9 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-dioleoyl-sn-glycero-3-phosphoserine | Arabidopsis thaliana |
| 14.4 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-dioctanoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 15.3 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-dioleoyl-sn-glycero-3-phosphorac-(1-glycerol) | Arabidopsis thaliana |
| 15.6 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | Arabidopsis thaliana |
| 16.5 | - |
purified recombinant AtsPLA2-alpha, substrate 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 16.7 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-dioleoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 17.8 | - |
purified recombinant AtsPLA2-alpha, substrate 1-oleoyl-2-stearoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 18 | - |
purified recombinant AtsPLA2-alpha, substrate 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 19.5 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-dilinoleoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 20.6 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-dipalmitoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 25.3 | - |
purified recombinant AtsPLA2-alpha, substrate 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 27.3 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-dimyristoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 29.3 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-dilauroyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
| 35.5 | - |
purified recombinant AtsPLA2-alpha, substrate 1,2-didecanoyl-sn-glycero-3-phosphocholine | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-diacyl-sn-glycero-3-phosphate + H2O | substrate from soybean | Arabidopsis thaliana | 1-acyl-sn-glycero-3-phosphate + a carboxylate | - |
? | |
| 1,2-diacyl-sn-glycero-3-phospho-(1-D-myo-inositol) + H2O | substrate from soybean | Arabidopsis thaliana | 1-acyl-sn-glycero-3-phospho-(1-D-myo-inositol) + a carboxylate | - |
? | |
| 1,2-diacyl-sn-glycero-3-phosphocholine + H2O | substrate from soybean | Arabidopsis thaliana | 1-acyl-sn-glycero-3-phosphocholine + a carboxylate | - |
? | |
| 1,2-didecanoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-decanoyl-sn-glycero-3-phosphocholine + decanoate | - |
? | |
| 1,2-diheptanoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-heptanoyl-sn-glycero-3-phosphocholine + heptanoate | - |
? | |
| 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O | low activity | Arabidopsis thaliana | 1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate | - |
? | |
| 1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-lauroyl-sn-glycero-3-phosphocholine + laurate | - |
? | |
| 1,2-dilinoleoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-linoleoyl-sn-glycero-3-phosphocholine + linoleoate | - |
? | |
| 1,2-dimyristoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-myristoyl-sn-glycero-3-phosphocholine + myristate | - |
? | |
| 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-octanoyl-sn-glycero-3-phosphocholine + octanoate | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phosphocholine + H2O | preferred substrate | Arabidopsis thaliana | 1-oleoyl-sn-glycero-3-phosphocholine + oleate | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine + H2O | - |
Arabidopsis thaliana | 1-oleoyl-sn-glycero-3-phosphoethanolamine + oleate | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phosphorac-(1-glycerol) + H2O | - |
Arabidopsis thaliana | 1-oleoyl-sn-glycero-3-phosphorac-(1-glycerol) + oleate | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phosphoserine + H2O | - |
Arabidopsis thaliana | 1-oleoyl-sn-glycero-3-phosphoserine + oleate | - |
? | |
| 1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-palmitoyl-sn-glycero-3-phosphocholine + palmitate | - |
? | |
| 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-oleoyl-sn-glycero-3-phosphocholine + palmitate | - |
? | |
| 1-oleoyl-2-stearoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-oleoyl-sn-glycero-3-phosphocholine + stearate | - |
? | |
| 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-palmitoyl-sn-glycero-3-phosphocholine + linoleate | - |
? | |
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol) + H2O | - |
Arabidopsis thaliana | 1-palmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) + oleate | - |
? | |
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O | - |
Arabidopsis thaliana | 1-palmitoyl-sn-glycero-3-phosphocholine + oleate | - |
? | |
| additional information | substrate specificity, overview, substrate application in micelles, no activity with 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine, AtsPLA2-alpha prefers zwitterionic phospholipids, and shows lower activity toward anionic phospholipids, in substrates with two identical fatty acid chains, AtsPLA2-alpha shows optimum activity toward phospholipids with decanoyl groups, in substrates with palmitoyl groups in sn-1 position, acyl chains with higher degree of unsaturation in sn-2 position are preferred, excluding arachidonic acid, overview | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtsPLA2-alpha | - |
Arabidopsis thaliana |
| secretory phospholipase A2-alpha | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 75 | activity range, temperature profile, overview | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.53 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | pH 8.5, 37°C, recombinant AtsPLA2-alpha, substrate below critical micelle concentration | Arabidopsis thaliana | |
| 1.71 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | pH 8.5, 37°C, recombinant AtsPLA2-alpha, substrate above critical micelle concentration or in presence of 2 M NaCl | Arabidopsis thaliana | |
| 3.86 | - |
1,2-diheptanoyl-sn-glycero-3-phosphocholine | pH 8.5, 37°C, recombinant AtsPLA2-alpha | Arabidopsis thaliana | |
| 4.38 | - |
1,2-dioctanoyl-sn-glycero-3-phosphocholine | pH 8.5, 37°C, recombinant AtsPLA2-alpha | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.5 | activity range, no activity below pH 6.5 and above pH 10.5, pH profile, overview | Arabidopsis thaliana |
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