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Literature summary for 3.1.1.4 extracted from

  • Gora, S.; Lambeau, G.; Bollinger, J.G.; Gelb, M.; Ninio, E.; Karabina, S.
    The proinflammatory mediator Platelet Activating Factor is an effective substrate for human group X secreted phospholipase A2 (2006), Biochim. Biophys. Acta, 1761, 1093-1099.
    View publication on PubMed
Search for more literature for 3.1.1.4

Protein Variants

Protein Variants Comment Organism
H48Q site-directed mutagenesis, inactive catalytic site mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine competitive inhibition of platelet activating factor hydrolysis Homo sapiens
EDTA 95% inhibition at 5 mM Homo sapiens
LY 329722 i.e. 3-(3-aminooxalyl-1-benzyl-2-ethyl-6-methyl-1H-indol-4-yl)propionic acid Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Homo sapiens
-
 1-acylglycerophosphocholine + a carboxylate
-
 ?
platelet activating factor + H2O Homo sapiens hGX sPLA2 hydrolyzes platelet activating factor, which is a reaction catalyzed by the platelet activating factor-acetylhydrolase, EC 3.1.1.47 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
 substrate 1-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine Homo sapiens
8.56
-
 substrate 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + H2O i.e. PAF or platelet activating factor Homo sapiens 1-hexadecanoyl-glycero-3-phosphoglycerol + acetate
-
 ?
1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-glycero-3-phosphocholine + arachidonate
-
 ?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O preferred substrate Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
additional information hGX sPLA2 prefers phosphatidylcholine 50fold to 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine as substrate, its hydrolyzing activity towards platelet activating factor rises abruptly when the concentration of the substrate passes through its critical micelle concentration, overview Homo sapiens ?
-
 ?
phosphatidylcholine + H2O
-
 Homo sapiens 1-acylglycerophosphocholine + a carboxylate
-
 ?
platelet activating factor + H2O hGX sPLA2 hydrolyzes platelet activating factor, which is a reaction catalyzed by the platelet activating factor-acetylhydrolase, EC 3.1.1.47 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
hGX sPLA2
-
 Homo sapiens
More the enzyme is a group X secreted phospholipase A2 Homo sapiens
PLA2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens