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Literature summary for 3.1.1.4 extracted from

  • Nemec, K.N.; Pande, A.H.; Qin, S.; Bieber Urbauer, R.J.; Tan, S.; Moe, D.; Tatulian, S.A.
    Structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2 (2006), Biochemistry, 45, 12448-12460.
    View publication on PubMed
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies Homo sapiens

Protein Variants

Protein Variants Comment Organism
F5W site-directed mutagenesis, mutation of the substrate binding pocket leads to reduced enzyme activity, the inhibitory effect of the F5W mutation results from the high affinity of Trp for the membrane-water interface Homo sapiens
V3W site-directed mutagenesis, mutation of the membrane binding surface residue results in substantial enhancement of the enzyme activity, the stimulatory effect of the V3W mutation results from the high affinity of Trp for the membrane-water interface Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and membrane binding of recombinant wild-type and mutant enzymes, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretory phospholipase A2 Homo sapiens
-
-
membrane hIBPLA2, structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2, overview Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant solubilized wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heparin affinity chromatography and gel filtration to homogeneity Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli inclusion bodies Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-bis-(10-pyrenedecanoyl)-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-(10-pyrenedecanoyl)-glycero-3-phosphocholine + 10-pyrenedecanoate
-
 ?
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphorylcholine + palmitate
-
 ?
1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol + H2O
-
 Homo sapiens 1-palmitoyl-glycero-3-phosphoglycerol + palmitate
-
 ?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphorylglycerol + oleate
-
 ?
1-palmitoyl-2-stearoyl-dibromo-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens ?
-
 ?
additional information structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
hIIAPLA2
-
 Homo sapiens
human group IIA phospholipase A2
-
 Homo sapiens
PLA2
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens