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Literature summary for 3.1.1.4 extracted from
- Purification, characterization, and molecular cloning of group I phospholipases A2 from the gills of the red sea bream, Pagrus major (2000), Lipids, 35, 1359-1370.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium cholate | stimulates, optimal concentration: 6-8 mM | Pagrus major |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isoform G-3 PLA2 | Pagrus major |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition at 1 mM | Pagrus major | |
| EGTA | complete inhibition at 1 mM | Pagrus major | |
| Sodium dodecyl sulfate | - |
Pagrus major | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requirement, optimal concentration: 2-40 mM | Pagrus major | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
SDS-PAGE | Pagrus major |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pagrus major | - |
red sea bream | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoforms G-1 PLA2, G-1 PLA2, G-3 PLA2 | Pagrus major |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | - |
Pagrus major | - |
| gill | highest activity | Pagrus major | - |
| heart | - |
Pagrus major | - |
| hepatopancreas | - |
Pagrus major | - |
| intestine | - |
Pagrus major | - |
| testis | - |
Pagrus major | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1143 | - |
isoform G-2 PLA2 | Pagrus major |
| 1293 | - |
isoform G-3 PLA2 | Pagrus major |
| 1294 | - |
isoform G-1 PLA2 | Pagrus major |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O | - |
Pagrus major | 1-palmitoyl-sn-glycero-3-phosphocholine + oleic acid | - |
? | |
| phosphatidylcholine + H2O | - |
Pagrus major | 1-acylglycerophosphocholine + fatty acid | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10 | - |
Pagrus major |
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