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Literature summary for 3.1.1.4 extracted from

  • Hanahan, D.J.
    Phospholipases (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 5, 71-85.
No PubMed abstract available
Search for more literature for 3.1.1.4

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Crotalus adamanteus

Inhibitors

Inhibitors Comment Organism Structure
Al3+ can replace Ca2+, decreased activity Crotalus adamanteus
Ba2+
-
 Crotalus adamanteus
Ba2+
-
 Rattus norvegicus
Ba2+
-
 Sus scrofa
Cd2+
-
 Sus scrofa
Co2+ can replace Ca2+, decreased activity Crotalus adamanteus
diisopropyl fluorophosphate
-
 Crotalus atrox
EDTA
-
 Rattus norvegicus
EDTA
-
 Sus scrofa
Fe2+
-
 Crotalus adamanteus
Fe2+
-
 Rattus norvegicus
Pb2+
-
 Sus scrofa
Zn2+
-
 Crotalus adamanteus
Zn2+
-
 Crotalus atrox
Zn2+
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.3
-
 lecithin in ether Crotalus atrox
8.5
-
 lecithin in chloroform Crotalus atrox

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Rattus norvegicus
Ca2+
-
 Sus scrofa
Ca2+
-
 Crotalus adamanteus
Ca2+
-
 Crotalus atrox
Cd2+ can replace Ca2+, decreased activity Crotalus adamanteus
Mg2+ can replace Ca2+, decreased activity Crotalus adamanteus
Mn2+ can replace Ca2+ but enzyme shows decreased activity Crotalus adamanteus

Organism

Organism UniProt Comment Textmining
Crotalus adamanteus
-
-
-
Crotalus atrox
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, column chromatography Crotalus atrox
column chromatography Sus scrofa
gel filtration, column chromatography Crotalus adamanteus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Sus scrofa
-
venom
-
 Crotalus adamanteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3150
-
 substrate: phosphatidylcholine Crotalus adamanteus
3250
-
 substrate: phosphatidylcholine Crotalus adamanteus

Storage Stability

Storage Stability Organism
4°C, for months without loss of activity Crotalus adamanteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphatidylglycerol + H2O
-
 Rattus norvegicus ?
-
 ?
phosphatidic acid + H2O
-
 Sus scrofa 1-acylglycerophosphate + fatty acid
-
 ?
phosphatidic acid + H2O
-
 Crotalus adamanteus 1-acylglycerophosphate + fatty acid
-
 ?
phosphatidylcholine + H2O
-
 Rattus norvegicus 1-acylglycerophosphocholine + fatty acid
-
 ?
phosphatidylcholine + H2O
-
 Sus scrofa 1-acylglycerophosphocholine + fatty acid
-
 ?
phosphatidylcholine + H2O
-
 Crotalus adamanteus 1-acylglycerophosphocholine + fatty acid
-
 ?
phosphatidylcholine + H2O
-
 Crotalus atrox 1-acylglycerophosphocholine + fatty acid
-
 ?
phosphatidylethanolamine + H2O
-
 Rattus norvegicus 1-acylglycerophosphorylethanolamine + fatty acid
-
 ?
phosphatidylethanolamine + H2O
-
 Sus scrofa 1-acylglycerophosphorylethanolamine + fatty acid
-
 ?
phosphatidylglycerol + H2O
-
 Rattus norvegicus 1-acylglycerol + fatty acid
-
 ?
phosphatidylglycerol + H2O
-
 Sus scrofa 1-acylglycerol + fatty acid
-
 ?
phosphatidylglycerol + H2O
-
 Crotalus adamanteus 1-acylglycerol + fatty acid
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 for 30 min., pH 3.0 Crotalus atrox
98
-
 for 5 min., pH 4.0, no loss of activity, for 1 h, pH 4.0, 45% of activity are recovered Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.7
-
 phosphatidylcholine
-
 Crotalus adamanteus