Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | - |
Escherichia coli |
General Stability | Organism |
---|---|
detergent-resistant enzyme, Triton X-100 prevents the enzymes thermal inactivation at 37øC | Escherichia coli |
sodium cholate prevents the enzymes thermal inactivation at 37øC | Escherichia coli |
sodium deoxycholate prevents the enzymes thermal inactivation at 37øC | Escherichia coli |
Span 40 prevents the enzymes thermal inactivation at 37C > | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
phosphatidylethanolamine | active enzyme | Escherichia coli | |
0.167 | - |
phosphatidylethanolamine | inactivated enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Escherichia coli | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
SDS-PAGE | Escherichia coli |
29000 | - |
SDS-PAGE, enzyme previously inactivated by heat treatment at 80°C or boiled in solution containing SDS | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospholipids + H2O | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Purification (Comment) | Organism |
---|---|
gel chromatography | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Escherichia coli | 1-acylglycerophosphocholine + fatty acid | - |
? | |
phosphatidylethanolamine + H2O | - |
Escherichia coli | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
phospholipids + H2O | - |
Escherichia coli | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 43 | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | aqueous solution of purified enzyme, with Triton X-100 | Escherichia coli |
37 | 70 | aqueous solution of purified enzyme, without Triton X-100 | Escherichia coli |
100 | - |
crude preparation, even activation observable | Escherichia coli |