Cloned (Comment) | Organism |
---|---|
into pBR322 and pCI1857, transformation of Escherichia coli strains HB101 and AB1157 | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
gel filtration, amino acid composition | Sus scrofa |
15000 | - |
- |
Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospholipids + H2O | Sus scrofa | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P00592 | - |
- |
Purification (Comment) | Organism |
---|---|
on CM ion exchange resin, over 90% pure | Sus scrofa |
Renatured (Comment) | Organism |
---|---|
S-sulfonation method | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
substrate: monomeric dihexanoyl-dithio-phosphatidylcholine, proPLA2 | Sus scrofa |
2000 | - |
substrate: micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, active PLA2 | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O | micellar substrate | Sus scrofa | 1-octanoyl-sn-glycero-3-phosphocholine + octanoate | - |
? | |
dihexanoyl-dithio-phosphatidylcholine + H2O | proPLA2, monomeric substrate | Sus scrofa | ? | - |
? | |
phospholipids + H2O | - |
Sus scrofa | ? | - |
? |