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Literature summary for 3.1.1.4 extracted from

  • De Geus, P.; van den Bergh, C.J.; Kuipers, O.; Verheij, H.M.; Hockstra, W.P.M.; de Haas, G.H.
    Expression of porcine pancreatic phospholipase A2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein from Escherichia coli (1987), Nucleic Acids Res., 15, 3743-3759.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
into pBR322 and pCI1857, transformation of Escherichia coli strains HB101 and AB1157 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
gel filtration, amino acid composition Sus scrofa
15000
-
-
Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Sus scrofa
-
?
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa P00592
-
-

Purification (Commentary)

Purification (Comment) Organism
on CM ion exchange resin, over 90% pure Sus scrofa

Renatured (Commentary)

Renatured (Comment) Organism
S-sulfonation method Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
substrate: monomeric dihexanoyl-dithio-phosphatidylcholine, proPLA2 Sus scrofa
2000
-
substrate: micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, active PLA2 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O micellar substrate Sus scrofa 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
?
dihexanoyl-dithio-phosphatidylcholine + H2O proPLA2, monomeric substrate Sus scrofa ?
-
?
phospholipids + H2O
-
Sus scrofa ?
-
?