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Literature summary for 3.1.1.4 extracted from
- Purification and characterization of a phospholipase A2 associated with rabbit lung microsomes: some evidence for its mitochondrial origin (1990), Biochim. Biophys. Acta, 1046, 258-266.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Oryctolagus cuniculus |  |
| EDTA | - |
Oryctolagus cuniculus | |
| iodoacetamide | weak inhibitor | Oryctolagus cuniculus | |
| N-ethylmaleimide | weak inhibitor | Oryctolagus cuniculus | |
| p-bromophenacyl bromide | - |
Oryctolagus cuniculus | |
| p-hydroxymercuribenzoate | weak inhibitor | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
1-palmitoyl-2-arachidonoylphosphatidylethanolamine | at pH 8.5, without detergents | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Oryctolagus cuniculus | 5764 | - |
| microsome | - |
Oryctolagus cuniculus | - |
- |
| mitochondrion | - |
Oryctolagus cuniculus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Oryctolagus cuniculus | |
| Cd2+ | 1% of the activity obtained with Ca2+ | Oryctolagus cuniculus | |
| Sr2+ | 1% of the activity obtained with Ca2+ | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 6500 | - |
gel filtration, underestimation seems obvious to authors | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipids + H2O | Oryctolagus cuniculus | inflammatory diseases | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange chromatography, gel-filtration chromatography, hydroxyapatite chromatography, 72500fold | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lung | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 290 | - |
substrate: 1-palmitoyl-2-oleolylphosphatidylcholine | Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in buffer A, 71 days, 4% decrease of activity | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dipalmitoyl-phosphatidylcholine + H2O | - |
Oryctolagus cuniculus | 1-palmitoyl-glycerophosphorylcholine + palmitic acid | - |
? | |
| 1-palmitoyl-2-arachidonoylphosphatidylethanolamine | - |
Oryctolagus cuniculus | 1-palmitoyl-glycerophosphorylethanolamine + arachidonic acid | - |
? | |
| 1-palmitoyl-2-oleoylphosphatidylcholine + H2O | - |
Oryctolagus cuniculus | 1-palmitoyl-glycerophosphocholine + oleic acid | - |
? | |
| phospholipids + H2O | inflammatory diseases | Oryctolagus cuniculus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
substrate: phosphatidylcholine | Oryctolagus cuniculus |
| 9.5 | 10 | substrate: phosphatidylethanolamine | Oryctolagus cuniculus |
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