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Literature summary for 3.1.1.4 extracted from
- Characterization of hormonally regulated and particulate-associated phospholipase A2 from bovine endothelial cells (1993), J. Biol. Chem., 268, 11697-11702.
General Stability
| General Stability | Organism |
|---|---|
| octyl glucoside, selective solubilization of hormonally regulated PLA2, stable | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Bos taurus | 5737 | - |
| particle-bound | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Bos taurus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 103000 | - |
centrifugation in sucrose density gradients | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipids + H2O | Bos taurus | influence on platelet aggregation | ? | - |
? | |
| phospholipids + H2O | Bos taurus | release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| subcellular fractionation | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endothelial cell | from pulmonary artery | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000115 | - |
particulate associated hormonally regulated PLA2, substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine, mixed micelles with Triton X-100 | Bos taurus |
| 0.0000538 | - |
particulate associated hormonally regulated PLA2, substrate: micellar 1-palmitoyl-2-arachidonylphosphatidylcholine | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, for up to 2 weeks without loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O | - |
Bos taurus | 1-palmitoyl-glycerophosphorylcholine + arachidonic acid | - |
? | |
| 1-palmitoyl-2-oleoylphosphatidylcholine + H2O | - |
Bos taurus | 1-palmitoyl-glycerophosphocholine + oleic acid | - |
? | |
| phospholipids + H2O | influence on platelet aggregation | Bos taurus | ? | - |
? | |
| phospholipids + H2O | release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins | Bos taurus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | higher activity at pH 8.5, barely detectable activity at pH 5 or 6 | Bos taurus |
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