Any feedback?
Literature summary for 3.1.1.4 extracted from
- Preparation and characterization of human rheumatoid arthritic synovial fluid phospholipase A2 produced by recombinant baculovirus-infected cells (1994), J. Biochem., 116, 82-87.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pAcYM1, transfer into baculovirus, transfection of Sf9 S.frugiperda cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Homo sapiens |  |
| sodium deoxycholate | - |
Homo sapiens | |
| Triton X-100 | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
phosphatidylethanolamine | recombinant PLA2 | Homo sapiens | |
| 0.024 | - |
phosphatidylethanolamine | natural PLA2 | Homo sapiens | |
| 0.05 | - |
phosphatidylcholine | recombinant PLA2 | Homo sapiens | |
| 0.051 | - |
phosphatidylcholine | natural PLA2 | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13700 | - |
natural PLA2, SDS-PAGE | Homo sapiens |
| 14100 | - |
recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipids + H2O | Homo sapiens | inflammatory diseases | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cation exchange column, reversed phase HPLC | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| synovia | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 110 | - |
recombinant enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine | Homo sapiens |
| 221 | - |
natural enzyme, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O | - |
Homo sapiens | 1-palmitoyl-glycerophosphorylethanolamine + linolic acid | - |
? | |
| phosphatidylethanolamine + H2O | - |
Homo sapiens | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
| phospholipids + H2O | inflammatory diseases | Homo sapiens | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | optimum at pH 9 | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
