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Literature summary for 3.1.1.4 extracted from
- Purification and characterization of a calcium-independent acidic phospholipase A2 from rat lung (1994), Biochem. J., 304, 131-137.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-hexadecyl-3-trifluoroethylglycero-sn-2-phosphomethanol | MJ33, competitive inhibitor | Rattus norvegicus |  |
| Ba2+ | - |
Rattus norvegicus | |
| Ca2+ | 30% decrease of activity at 5-10 mM | Rattus norvegicus | |
| Mg2+ | - |
Rattus norvegicus | |
| surfactant protein A | - |
Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lamellar body | - |
Rattus norvegicus | 42599 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
gel filtration, gel electrophoresis, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipids + H2O | Rattus norvegicus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 560fold | Rattus norvegicus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lung | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.04 | - |
renatured enzyme, substrate: 1,2-dipalmitoyl-phosphatidylcholine | Rattus norvegicus |
| 0.56 | - |
native enzyme, substrate: 1,2-dipalmitoyl-phosphatidylcholine | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, activity increased 3fold after storage for one week | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dipalmitoyl-phosphatidylcholine + H2O | - |
Rattus norvegicus | 1-palmitoyl-glycerophosphorylcholine + palmitic acid | - |
? | |
| phosphatidylcholine + H2O | - |
Rattus norvegicus | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| phospholipids + H2O | - |
Rattus norvegicus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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