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Literature summary for 3.1.1.4 extracted from

  • Rice, S.Q.J.; Southan, C.; Boyd, H.F.; Terrett, J.A.; MacPhee, C.H.; Moores, K.; Gloger, I.S.; Tew, D.G.
    Expression, purification and characterization of a human serine-dependent phospholipase A2 with high specificity for oxidized phospholipids and platelet activating factor (1998), Biochem. J., 330, 1309-1315.
No PubMed abstract available
Search for more literature for 3.1.1.4

Activating Compound

Activating Compound Comment Organism Structure
serine human serine dependent PLA2 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into baculovirus, transfection of SF9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin
-
 Homo sapiens
Diethyl p-nitrophenyl phosphate
-
 Homo sapiens
diisopropyl fluorophosphate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 1-decanoyl-2-(4-nitrophenylglutaryl) phosphate
-
 Homo sapiens
0.042
-
 platelet activating factor
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44160
-
 SDS-PAGE and liquid chromatography Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Homo sapiens influence on platelet aggregation ?
-
 ?
platelet activating factor + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
leukocyte peripheral blood Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35
-
 HSD-PLA2, 21fold purification, platelet activating factor for substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-decanoyl-2-(4-nitrophenylglutaryl)glycerol 3-phosphate + H2O HSD-PLA2 Homo sapiens ?
-
 ?
oxidized phosphatidylcholines + H2O HSD-PLA2 Homo sapiens 1-acylglycerophosphocholine + oxidized fatty acids
-
 ?
phospholipids + H2O influence on platelet aggregation Homo sapiens ?
-
 ?
platelet activating factor + H2O
-
 Homo sapiens ?
-
 ?
platelet activating factor + H2O PAF i.e. platelet activating factor, HSD-PLA2 Homo sapiens ?
-
 ?