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Literature summary for 3.1.1.32 extracted from
- Purification and properties of phospholipase A1 from bovine brain (1994), J. Biol. Chem., 269, 19494-19500.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CHAPS | - |
Bos taurus |  |
| octyl-beta-glucoside | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal stimulation at 1 mM | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 365000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, indefinitely stable | Bos taurus |
| 4°C, stable for at least 2 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-palmitoyl-2-arachidonoyl-glycero-3-phosphoethanolamine + H2O | - |
Bos taurus | 2-arachidonoyl-lysophosphatidylethanolamine + H2O | - |
? | |
| 1-palmitoyl-2-arachidonoylglycerophosphocholine + H2O | - |
Bos taurus | palmitic acid + 2-arachidonoylglycerophosphocholine | - |
? | |
| 1-palmitoyl-2-palmitoylglycerophosphocholine + H2O | - |
Bos taurus | palmitic acid + 2-palmitoylglycerophosphocholine | - |
? | |
| 1-stearoyl-2-arachidonoylglycerophosphocholine + H2O | - |
Bos taurus | stearic acid + 2-arachidonoylglycerophosphocholine | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, more than 90% loss of activity | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 9.3 | pH 7.8: about 55% of maximal activity, pH 9.3: 90% of maximal activity | Bos taurus |
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Release 2023.1 (January 2023)
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