Literature summary for 3.1.1.32 extracted from

Borodina, I.; Jensen, B.M.; Wagner, T.; Hachem, M.A.; S?ndergaard, I.; Poulsen, L.K.
Expression of enzymatically inactive wasp venom phospholipase A1 in Pichia pastoris (2011), PLoS ONE, 6, e21267.

Search for more literature for 3.1.1.32

Application

Application	Comment	Organism
pharmacology	the recombinant protein is allergenic in a biological assay as demonstrated by its capability to induce histamine release of wasp venom-sensitive basophils. The recombinant phospholipase A1 presents a good candidate for wasp venom immunotherapy	Vespula vulgaris

Cloned(Commentary)

Cloned (Comment)	Organism
genes Vesv1 and Vesv5, subcloning in Escherichia coli strain Dh5alpha, recombinant expression of His-tagged Vesv5 and Vesv1 wild-type and His-tagged Vesv1 mutant enzymes in Pichia pastoris strains X33 and KM71H as secreted proteins, method optimization, overview	Vespula vulgaris

Protein Variants

Protein Variants	Comment	Organism
D165A	site-directed mutagenesis of gene Vesv1	Vespula vulgaris
H229A	site-directed mutagenesis of gene Vesv1, inactive mutant, that binds IgE antibodies from wasp venom allergic patients and inhibits the binding of wasp venom to IgE antibodies specific for phospholipase A1. The recombinant mutant protein is allergenic in a biological assay as demonstrated by its capability to induce histamine release of wasp venom-sensitive basophils	Vespula vulgaris
S137A	site-directed mutagenesis of gene Vesv1	Vespula vulgaris

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	venom	Vespula vulgaris	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	x * 36000, SDS-PAGE	Vespula vulgaris

Organism

Organism	UniProt	Comment	Textmining
Vespula vulgaris	-	genes Vesv1 and Vesv5	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged Vesv5 and Vesv1 wild-type and His-tagged Vesv1 mutant enzymes from Pichia pastoris strains X33 and KM71H from culture supernatant	Vespula vulgaris

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Vespula vulgaris	-

Subunits

Subunits	Comment	Organism
?	x * 36000, SDS-PAGE	Vespula vulgaris

General Information

General Information	Comment	Organism
physiological function	PLA1 is the major allergen for humans in the wasp venom	Vespula vulgaris

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Release 2023.1 (January 2023)