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Literature summary for 3.1.1.32 extracted from

  • Uyama, T.; Morishita, J.; Jin, X.H.; Okamoto, Y.; Tsuboi, K.; Ueda, N.
    The tumor suppressor gene H-Rev107 functions as a novel Ca2+-independent cytosolic phospholipase A1/2 of the thiol hydrolase type (2009), J. Lipid Res., 50, 685-693.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Mus musculus
dithiothreitol
-
Homo sapiens
dithiothreitol required for full activity. Addition of 2 mM increases PLA1/2 activity 167fold Rattus norvegicus
additional information purified H-Rev107 exhibits an 88fold higher PLA1/2 activity than the cytosolic fraction Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells Mus musculus
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells Homo sapiens
expression vector harboring H-Rev107 with a FLAG tag at the N terminus. Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information deletion mutant of H-Rev107 lacking either N-terminal 8 or N-terminal 16 amino acids (DELTAN1, DELTAN2) or C-terminal 25 amino acids (DELTAC1). Expression of the mutants with the expected molecular masses. As for PLA1/2 activity, DELTAN1 shows very low activity (about 3% that of the wild-type), and DELTAN2 and DELTAC1 are entirely inactive Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid
-
Homo sapiens
iodoacetic acid
-
Mus musculus
iodoacetic acid
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mus musculus 5829
-
cytosol
-
Homo sapiens 5829
-
cytosol
-
Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2 Mus musculus
additional information H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2 Homo sapiens
additional information H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
x * 19000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus P53817
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus
-
Homo sapiens
FLAG-tagged H-Rev107 by immunoprecipitation Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
testis
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dipalmitoylphosphatidylcholine + H2O
-
Mus musculus palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
?
dipalmitoylphosphatidylcholine + H2O
-
Homo sapiens palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
?
dipalmitoylphosphatidylcholine + H2O
-
Rattus norvegicus palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
?
additional information His21 and Cys111 of H-Rev107 form a catalytic dyad. The N-terminal proline-rich and C-terminal hydrophobic domains are involved in the catalytic activity and/or proper folding Rattus norvegicus ?
-
?
phosphatidylcholine + H2O
-
Mus musculus 2-acylglycerophosphocholine + a carboxylate
-
?
phosphatidylcholine + H2O
-
Homo sapiens 2-acylglycerophosphocholine + a carboxylate
-
?
phosphatidylcholine + H2O
-
Rattus norvegicus 2-acylglycerophosphocholine + a carboxylate
-
?
phosphatidylethanolamine + H2O
-
Mus musculus 2-acylglycerophosphoethanolamine + a carboxylate
-
?
phosphatidylethanolamine + H2O
-
Homo sapiens 2-acylglycerophosphoethanolamine + a carboxylate
-
?
phosphatidylethanolamine + H2O
-
Rattus norvegicus 2-acylglycerophosphoethanolamine + a carboxylate
-
?

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
H-Rev107
-
Mus musculus
H-Rev107
-
Homo sapiens
H-Rev107
-
Rattus norvegicus
phospholipase A1/2
-
Mus musculus
phospholipase A1/2
-
Homo sapiens
phospholipase A1/2
-
Rattus norvegicus
PLA1/2
-
Mus musculus
PLA1/2
-
Homo sapiens
PLA1/2
-
Rattus norvegicus

General Information

General Information Comment Organism
physiological function H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation Mus musculus
physiological function H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation Homo sapiens
physiological function H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation Rattus norvegicus