Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Mus musculus | |
dithiothreitol | - |
Homo sapiens | |
dithiothreitol | required for full activity. Addition of 2 mM increases PLA1/2 activity 167fold | Rattus norvegicus | |
additional information | purified H-Rev107 exhibits an 88fold higher PLA1/2 activity than the cytosolic fraction | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells | Mus musculus |
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells | Homo sapiens |
expression vector harboring H-Rev107 with a FLAG tag at the N terminus. Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutant of H-Rev107 lacking either N-terminal 8 or N-terminal 16 amino acids (DELTAN1, DELTAN2) or C-terminal 25 amino acids (DELTAC1). Expression of the mutants with the expected molecular masses. As for PLA1/2 activity, DELTAN1 shows very low activity (about 3% that of the wild-type), and DELTAN2 and DELTAC1 are entirely inactive | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetic acid | - |
Homo sapiens | |
iodoacetic acid | - |
Mus musculus | |
iodoacetic acid | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2 | Mus musculus | |
additional information | H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2 | Homo sapiens | |
additional information | H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2 | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
x * 19000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | P53817 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
- |
Homo sapiens |
FLAG-tagged H-Rev107 by immunoprecipitation | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
testis | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dipalmitoylphosphatidylcholine + H2O | - |
Mus musculus | palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine | - |
? | |
dipalmitoylphosphatidylcholine + H2O | - |
Homo sapiens | palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine | - |
? | |
dipalmitoylphosphatidylcholine + H2O | - |
Rattus norvegicus | palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine | - |
? | |
additional information | His21 and Cys111 of H-Rev107 form a catalytic dyad. The N-terminal proline-rich and C-terminal hydrophobic domains are involved in the catalytic activity and/or proper folding | Rattus norvegicus | ? | - |
? | |
phosphatidylcholine + H2O | - |
Mus musculus | 2-acylglycerophosphocholine + a carboxylate | - |
? | |
phosphatidylcholine + H2O | - |
Homo sapiens | 2-acylglycerophosphocholine + a carboxylate | - |
? | |
phosphatidylcholine + H2O | - |
Rattus norvegicus | 2-acylglycerophosphocholine + a carboxylate | - |
? | |
phosphatidylethanolamine + H2O | - |
Mus musculus | 2-acylglycerophosphoethanolamine + a carboxylate | - |
? | |
phosphatidylethanolamine + H2O | - |
Homo sapiens | 2-acylglycerophosphoethanolamine + a carboxylate | - |
? | |
phosphatidylethanolamine + H2O | - |
Rattus norvegicus | 2-acylglycerophosphoethanolamine + a carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
H-Rev107 | - |
Mus musculus |
H-Rev107 | - |
Homo sapiens |
H-Rev107 | - |
Rattus norvegicus |
phospholipase A1/2 | - |
Mus musculus |
phospholipase A1/2 | - |
Homo sapiens |
phospholipase A1/2 | - |
Rattus norvegicus |
PLA1/2 | - |
Mus musculus |
PLA1/2 | - |
Homo sapiens |
PLA1/2 | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation | Mus musculus |
physiological function | H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation | Homo sapiens |
physiological function | H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation | Rattus norvegicus |