Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.32 extracted from

  • Morikawa, R.K.; Aoki, J.; Kano, F.; Murata, M.; Yamamoto, A.; Tsujimoto, M.; Arai, H.
    Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex (2009), J. Biol. Chem., 284, 26620-26630.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.32

Cloned(Commentary)

Cloned (Comment) Organism
polypeptide (amino acid number 368-486 of intracellular PLA1gamma) expressed as a glutathione S-transferase fusion protein in Escherichia coli. Purified protein used to immunize rats. The cDNA encoding intracellular PLA1gamma cloned into pEGFP-C3 vector and stably expressed in CHO cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
Golgi complex cis-Golgi and endoplasmic reticulum-Golgi intermediate compartment (ERGIC). Endogenous intracellular PLA1gamma does not appear to be localized to the ERGIC at 37°C. At 15°C, intracellular PLA1gamma shows marked colocalization with ERGIC-53 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
phospholipase A1gamma
-
 Homo sapiens
PLA1gamma
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens expression of intracellular PLA1gamma is suppressed by transfecting cells with siRNA against intracellular PLA1gamma. Expression of intracellular PLA1gamma protein is decreased to less than 10% that of control siRNA-treated cells down

General Information

General Information Comment Organism
malfunction knockdown of intracellular PLA1gamma expression by RNAi does not affect the anterograde transport of ts045 vesicular stomatitis virus protein (VSVGts045) but dramatically delays two types of Golgi-to-endoplasmic reticulum retrograde membrane transport, i.e., transfer of the Golgi membrane into the endoplasmic reticulum in the presence of brefeldin A and delivery of cholera toxin B subunit from the Golgi complex to the endoplasmic reticulum. Knockdown of intracellular PLA1gamma does not impair coat protein complex I- and Rab6-dependent retrograde transports represented by ERGIC-53 recycling and endoplasmic reticulum delivery of Shiga toxin, respectively Homo sapiens
physiological function mediates a specific retrograde membrane transport pathway between the endoplasmic reticulum and the Golgi complex Homo sapiens