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Literature summary for 3.1.1.32 extracted from

  • Song, J.K.; Rhee, J.S.
    Simultaneous enhancement of thermostability and catalytic activity of phospholipase A1 by evolutionary molecular engineering (2000), Appl. Environ. Microbiol., 66, 890-894.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Serratia sp.
expression of wild-type and PCR evolved phospholipase A1 variants in Escherichia coli Serratia sp.

Protein Variants

Protein Variants Comment Organism
additional information stability and catalytic activity of phospholipase A1 in the presence of an organic solvent is enhanced by error-prone polymerase chanin reaction and DNA shuffling followed by a filter-based visual screening Serratia sp.
additional information thermal stability and catalytic activity of phospholipase A1 are improved by evolutionary molecular engineering Serratia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 phosphatidylcholine 37°C, pH 8.0 Serratia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Serratia sp.
-
 2-acylglycerophosphocholine + a carboxylate
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitril 50%, approx. 90% loss of activity after 10 h Serratia sp.
butyl acetate 50%, approx. 80% loss of activity after 10 h Serratia sp.
dimethyl sulfoxide 50%, almost complete loss of activity after 10 h, approx. 90 min half-life Serratia sp.
hexane 50%, approx. 85% loss of activity after 10 h Serratia sp.
N,N-dimethylformamide 50%, almost complete loss of activity after 10 h Serratia sp.
octane 50%, approx. 82% loss of activity after 10 h Serratia sp.
toluene 50%, approx. 80% loss of activity after 10 h Serratia sp.

Organism

Organism UniProt Comment Textmining
Serratia sp. Q9X9D8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged phospholipase A1 Serratia sp.
recombinant phospholipase A1 Serratia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dimyristoyl-sn-glycero-3-phosphocholine + H2O
-
 Serratia sp. 2-myristoyl-sn-glycero-3-phosphocholine + myristic acid
-
 ?
phosphatidylcholine + H2O
-
 Serratia sp. 2-acylglycerophosphocholine + a carboxylate
-
 ?

Synonyms

Synonyms Comment Organism
phospholipase A1
-
 Serratia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 loss of 50% activity after 20 min Serratia sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.057 0.65 phosphatidylcholine 37°C, pH 8.0 Serratia sp.