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Literature summary for 3.1.1.3 extracted from
- Purification, crystallization and properties of triacylglycerol lipase from Pseudomonas fluorescens (1977), Biochim. Biophys. Acta, 488, 353-358.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | FeCl3, partial | Pseudomonas fluorescens |  |
| Iodine | - |
Pseudomonas fluorescens | |
| N-bromosuccinimide | - |
Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | increases activity | Pseudomonas fluorescens | |
| Ca2+ | CaCl2 increases activity | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
gel filtration | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trihexanoin + H2O | - |
Pseudomonas fluorescens | hexanoic acid + ? | - |
? | |
| tripalmitin + H2O | - |
Pseudomonas fluorescens | palmitic acid + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 5.0-11.0, 60 min, stable | Pseudomonas fluorescens |
| 40 | - |
pH 7.0, 30 min, stable up to | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | 37°C, 60 min, less than 20% loss of activity | Pseudomonas fluorescens |
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