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Literature summary for 3.1.1.3 extracted from

  • Li, X.; Benning, C.; Kuo, M.H.
    Rapid triacylglycerol turnover in Chlamydomonas reinhardtii requires a lipase with broad substrate specificity (2012), Eukaryot. Cell, 11, 1451-1462.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus cells Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
triacylglycerol + H2O Chlamydomonas reinhardtii
-
 diacylglycerol + a carboxylate
-
 ?
triacylglycerol + H2O Chlamydomonas reinhardtii dw15.1
-
 diacylglycerol + a carboxylate
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii dw15.1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diacylglycerol-N,N,N-trimethylhomoserine + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
digalactosyldiacylglycerol + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
monogalactosyldiacylglycerol + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
monogalactosyldiacylglycerol + H2O
-
 Chlamydomonas reinhardtii dw15.1 ?
-
 ?
olive oil + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
olive oil + H2O
-
 Chlamydomonas reinhardtii dw15.1 ?
-
 ?
phosphatidylethanolamine + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
phosphatidylethanolamine + H2O
-
 Chlamydomonas reinhardtii dw15.1 ?
-
 ?
phosphatidylglycerol + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
phosphatidylglycerol + H2O
-
 Chlamydomonas reinhardtii dw15.1 ?
-
 ?
triacylglycerol + H2O
-
 Chlamydomonas reinhardtii diacylglycerol + a carboxylate
-
 ?
triacylglycerol + H2O
-
 Chlamydomonas reinhardtii dw15.1 diacylglycerol + a carboxylate
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
Lip1
-
 Chlamydomonas reinhardtii
TAGL
-
 Chlamydomonas reinhardtii

General Information

General Information Comment Organism
metabolism the enzyme facilitates triacylglycerol turnover in Chlamydomonas primarily by degrading the diacylglycerol presumably generated from triacylglycerol hydrolysis Chlamydomonas reinhardtii