Application | Comment | Organism |
---|---|---|
biofuel production | biodiesel production | Haloarcula sp. |
General Stability | Organism |
---|---|
highly stable in the presence of 2.5-25% NaCl | Haloarcula sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, 7.7% inhibition | Haloarcula sp. | |
Cu2+ | 1 mM, 19% inhibition | Haloarcula sp. | |
diethyldicarbonate | 10 mM, 17% inhibition | Haloarcula sp. | |
EDTA | 10 mM, 92% inhibition | Haloarcula sp. | |
Hg2+ | 1 mM, 89% inhibition | Haloarcula sp. | |
PMSF | 10 mM, 89% inhibition | Haloarcula sp. | |
poly-alpha-olefin | 10 mM, 81% inhibition | Haloarcula sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Haloarcula sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 1.3fold stimulation | Haloarcula sp. | |
Mg2+ | 1 mM, 1.3fold stimulation | Haloarcula sp. | |
NaCl | no activity is detected in the absence of NaCl, optimal activity at 15% | Haloarcula sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Haloarcula sp. |
Organic Solvent | Comment | Organism |
---|---|---|
1-decanol | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
Acetone | 30% (v/v), half-life: 1 day, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
acetonitrile | 30% (v/v), half-life: less than 1 day, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
benzene | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
chloroform | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
cyclohexane | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
dimethylformamide | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
DMSO | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
Ethanol | 30% (v/v), half-life: less than 1 day, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
Glycerol | 30% (v/v), half-life: 4 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
isooctane | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
Methanol | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
n-hexane | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
tert-Butanol | 30% (v/v), half-life: 1 day, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
toluene | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula sp. | - |
- |
- |
Haloarcula sp. G41 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Haloarcula sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | 12% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | 12% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | 20% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | 20% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | 85% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl decanoate + H2O | 85% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | 30% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | 30% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl laurate + H2O | 95% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | maximal activity among the tested 4-nitrophenyl esters | Haloarcula sp. | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl octanoate + H2O | 70% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | 70% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl palmitate + H2O | 95% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | 90% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + stearate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Haloarcula sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Haloarcula sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | 30°C: about 70% of maximal activity, 80°C: about 70% of maximal activity | Haloarcula sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
24 h, stable | Haloarcula sp. |
90 | - |
24 h, 40% loss of activity | Haloarcula sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Haloarcula sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | pH 6.0: about 55% of maximal activity, pH 11.0: about 50% of maximal activity | Haloarcula sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | ighly stable in the pH range of 7.011.0 after 24 h incubation | Haloarcula sp. |