Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Colipase | 113% activity at 0.01 mM | Macruronus novaezelandiae | |
additional information | not influenced by colipase and potassium iodide | Oncorhynchus tshawytscha | |
sodium cholate | absolutely necessary both for lipase stability in the gel and for optimum activity, 5 mM improves the lipase activity approximately 100fold | Oncorhynchus tshawytscha | |
taurocholate | absolutely necessary both for lipase stability in the gel and for optimum activity, 10 mM improves the lipase activity approximately 130fold | Oncorhynchus tshawytscha |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 7% residual activity at 10 mM | Macruronus novaezelandiae | |
Fe2+ | 14% residual activity at 10 mM | Oncorhynchus tshawytscha | |
Hg2+ | complete inhibition at 1 mM | Macruronus novaezelandiae | |
Hg2+ | complete inhibition at 1 mM | Oncorhynchus tshawytscha | |
Mg2+ | 86% residual activity at 1 mM | Macruronus novaezelandiae | |
Mg2+ | 80% residual activity at 1 mM | Oncorhynchus tshawytscha | |
NaN3 | 78% residual activity at 10 mM | Macruronus novaezelandiae | |
NaN3 | 75% residual activity at 1 mM | Oncorhynchus tshawytscha | |
paraoxon | - |
Macruronus novaezelandiae | |
paraoxon | - |
Oncorhynchus tshawytscha | |
phenylmethylsulfonyl fluoride | 54% residual activity at 5 mM | Macruronus novaezelandiae | |
phenylmethylsulfonyl fluoride | 36% residual activity at 5 mM | Oncorhynchus tshawytscha | |
Triton X-100 | complete inhibition at 10% (v/v) | Macruronus novaezelandiae | |
Triton X-100 | complete inhibition at 10% (v/v) | Oncorhynchus tshawytscha | |
Tween 80 | 20% residual activity at 10% (v/v) | Macruronus novaezelandiae | |
Tween 80 | 33% residual activity at 10% (v/v) | Oncorhynchus tshawytscha | |
Zn2+ | 49% residual activity at 10 mM | Macruronus novaezelandiae | |
Zn2+ | 51% residual activity at 10 mM | Oncorhynchus tshawytscha |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
4-nitrophenyl caprate | pH 8.0, 30°C | Macruronus novaezelandiae | |
0.078 | - |
4-nitrophenyl caprate | pH 8.0, 30°C | Oncorhynchus tshawytscha |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolutely necessary both for lipase stability in the gel and for optimum activity | Macruronus novaezelandiae | |
Ca2+ | absolutely necessary both for lipase stability in the gel and for optimum activity, improves the lipase activity approximately 100fold | Oncorhynchus tshawytscha | |
Mn2+ | 113% activity at 10 mM | Macruronus novaezelandiae | |
Mn2+ | 143% activity at 10 mM | Oncorhynchus tshawytscha |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, calculated from SDS-PAGE | Macruronus novaezelandiae |
54900 | - |
x * 54900, final enzyme form, calculated from SDS-PAGE | Oncorhynchus tshawytscha |
79600 | - |
x * 79600, uncleaved enzyme form, calculated from SDS-PAGE | Oncorhynchus tshawytscha |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | complete inhibition at 50% (v/v) | Oncorhynchus tshawytscha |
Acetone | complete inhibition at 50% (v/v) | Macruronus novaezelandiae |
hexane | 82% residual activity at 100% (v/v) | Oncorhynchus tshawytscha |
hexane | 90% residual activity at 100% (v/v) | Macruronus novaezelandiae |
isooctane | 58% residual activity at 100% (v/v) | Oncorhynchus tshawytscha |
isooctane | 71% residual activity at 100% (v/v) | Macruronus novaezelandiae |
isopropanol | 23% residual activity at 50% (v/v) | Oncorhynchus tshawytscha |
isopropanol | 36% residual activity at 50% (v/v) | Macruronus novaezelandiae |
Methanol | complete inhibition at 50% (v/v) | Oncorhynchus tshawytscha |
Methanol | complete inhibition at 50% (v/v) | Macruronus novaezelandiae |
n-heptane | 103% activity at 100% (v/v) | Macruronus novaezelandiae |
n-heptane | 95% residual activity at 100% (v/v) | Oncorhynchus tshawytscha |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macruronus novaezelandiae | - |
New Zealand hoki | - |
Oncorhynchus tshawytscha | - |
Chinook salmon | - |
Purification (Comment) | Organism |
---|---|
fractional precipitation with polyethylene glycol 1000, cholate-Affi-Gel 102 affinity chromatography, and Sephacryl S-300 gel filtration | Oncorhynchus tshawytscha |
fractional precipitation with polyethylene glycol 1000, cholate-Affi-Gel 102 affinity chromatography, and Sephacryl S-300 gel filtration | Macruronus novaezelandiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pyloric cecum | - |
Oncorhynchus tshawytscha | - |
pyloric cecum | - |
Macruronus novaezelandiae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.36 | - |
crude extract, pH 8.0, 30°C | Oncorhynchus tshawytscha |
4.8 | - |
after 14fold purification pH 8.0, 30°C | Oncorhynchus tshawytscha |
4.8 | - |
after 14fold purification pH 8.0, 30°C | Macruronus novaezelandiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl butyrate + H2O | - |
Oncorhynchus tshawytscha | 4-methylumbelliferone + butyrate | - |
? | |
4-methylumbelliferyl butyrate + H2O | - |
Macruronus novaezelandiae | 4-methylumbelliferone + butyrate | - |
? | |
4-methylumbelliferyl oleate + H2O | - |
Oncorhynchus tshawytscha | 4-methylumbelliferone + oleate | - |
? | |
4-methylumbelliferyl oleate + H2O | - |
Macruronus novaezelandiae | 4-methylumbelliferone + oleate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Oncorhynchus tshawytscha | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Macruronus novaezelandiae | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl caprate + H2O | best substrate | Oncorhynchus tshawytscha | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprate + H2O | best substrate | Macruronus novaezelandiae | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprate + H2O | highest lipase activity is obtained with 0.25 mM of the substrate | Oncorhynchus tshawytscha | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprate + H2O | highest lipase activity is obtained with 0.25 mM of the substrate | Macruronus novaezelandiae | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl palmitate + H2O | highest lipase activity is obtained with 0.25 mM of the substrate | Oncorhynchus tshawytscha | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | highest lipase activity is obtained with 0.25 mM of the substrate | Macruronus novaezelandiae | 4-nitrophenol + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, calculated from SDS-PAGE | Macruronus novaezelandiae |
? | x * 54900, final enzyme form, calculated from SDS-PAGE | Oncorhynchus tshawytscha |
? | x * 79600, uncleaved enzyme form, calculated from SDS-PAGE | Oncorhynchus tshawytscha |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Oncorhynchus tshawytscha |
lipase | - |
Macruronus novaezelandiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Oncorhynchus tshawytscha |
35 | - |
- |
Macruronus novaezelandiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Oncorhynchus tshawytscha |
8 | 8.5 | - |
Macruronus novaezelandiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
paraoxon | pH 8.0, 30°C | Oncorhynchus tshawytscha | |
0.00517 | - |
paraoxon | pH 8.0, 30°C | Macruronus novaezelandiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oncorhynchus tshawytscha | isoelectric focusing | - |
5.7 |
Macruronus novaezelandiae | isoelectric focusing | - |
5.8 |