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Literature summary for 3.1.1.3 extracted from

  • Yesiloglu, Y.; Sit, L.
    Biochemical properties of free and immobilized Candida rugosa lipase onto Al2O3: a comparative study (2011), Artif. Cells Blood Substit. Immobil. Biotechnol., 39, 247-251.
    View publication on PubMed
Search for more literature for 3.1.1.3

Organism

Organism UniProt Comment Textmining
Diutina rugosa
-
-
-

Storage Stability

Storage Stability Organism
4°C, Al2O3-immobilized lipase in 50 mM phosphate buffer (pH 8.0), the immobilized lipase retains 90% of its catalytic activity Diutina rugosa
4°C, free enzyme in 50 mM phosphate buffer (pH 8.0), over a period of 4 months, the activity o the free enzyme gradually decreases to 50% Diutina rugosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme uses olive oil as substrate Diutina rugosa ?
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Diutina rugosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 free enzyme Diutina rugosa
40
-
 Al2O3-immobilized enzyme Diutina rugosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5 70 the free enzyme shows full activity after 1 h at 5°C, about 80% activity at 25-35°C, about 65% activity at 40-45°C, about 50% activity at 50°C and is completely inactive after 1 h at 60°C. The immobilized lipase holds approximately 30% activity at 55°C, while the free lipase remains less than 10% Diutina rugosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 free enzyme Diutina rugosa
8.5
-
 Al2O3-immobilized enzyme Diutina rugosa