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Literature summary for 3.1.1.3 extracted from

  • Chandrayan, S.K.; Dhaunta, N.; Guptasarma, P.
    Expression, purification, refolding and characterization of a putative lysophospholipase from Pyrococcus furiosus: retention of structure and lipase/esterase activity in the presence of water-miscible organic solvents at high temperatures (2008), Protein Expr. Purif., 59, 327-333.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
biofuel the high organic solvent stability and thermal stability suggest that this enzyme may have useful biodiesel-related applications Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
phenylmethylsulfonyl fluoride remarkable reduction in activity at 1 mM Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
recombinant protein, approximately 28000-29000 Da, SDS-PAGE Pyrococcus furiosus
31980
-
recombinant protein, MALDI-TOF spectrometry Pyrococcus furiosus
32040
-
recombinant protein, calculated from amino acid sequence Pyrococcus furiosus
82000
-
dynamic light scattering Pyrococcus furiosus

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile over 50% activity is retained at 70°C in the presence of 25% (v/v) acetonitrile Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U3I6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Pyrococcus furiosus

Renatured (Commentary)

Renatured (Comment) Organism
through heating at 80°C for 30 min in the presence of 6 M Gdm-HCl and 1 M NaCl Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Pyrococcus furiosus 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Pyrococcus furiosus 4-nitrophenol + butyrate
-
?
4-nitrophenyl palmitate + H2O
-
Pyrococcus furiosus 4-nitrophenol + palmitate
-
?

Subunits

Subunits Comment Organism
dimer or trimer in aqueous solution, dynamic light scattering Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
putative lysophospholipase the enzyme is confirmed to be an esterase and also shown to be a lipase (hydrolyzing 4-nitrophenyl palmitate), with lipolytic activity being overall about 18 to 20fold lower than esterase activity Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
using 4-nitrophenyl palmitate as a substrate, for a fixed pH of 8.0 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80
-
Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85 95 the lysophospholipase begins to unfold only above 85°C, with unfolding remaining incomplete even at 95°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
using 4-nitrophenyl palmitate as a substrate, for a fixed temperature at 70°C Pyrococcus furiosus