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Literature summary for 3.1.1.3 extracted from
- Cloning, purification and characterization of two lipases from Streptomyces coelicolor A3(2) (2008), Enzyme Microb. Technol., 42, 381-388.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | isoyzme SCO1725 shows 11% residual activity in the presence of 1 mM Ag+; isozyme SCO7513 shows 10% residual activity in the presence of 1 mM Ag+ | Streptomyces coelicolor |  |
| Ca2+ | isoyzme SCO1725 shows 91% residual activity at 10 mM Ca2+; isozyme SCO7513 shows 86% residual activity in the presence of 1 mM Ca2+ | Streptomyces coelicolor | |
| Co2+ | isoyzme SCO1725 shows 93% residual activity in the presence of 10 mM Co2+; isozyme SCO7513 shows 72% residual activity in the presence of 10 mM Co2+ | Streptomyces coelicolor | |
| Cu2+ | isoyzme SCO1725 shows 88% residual activity at 1 mM Cu2+; isozyme SCO7513 shows 51% residual activity in the presence of 10 mM Cu2+ | Streptomyces coelicolor | |
| dithiothreitol | isoyzme SCO1725 shows 36% residual activity in the presence of 10 mM dithiothreitol; isozyme SCO7513 shows 86% residual activity in the presence of 10 mM dithiothreitol | Streptomyces coelicolor | |
| Mg2+ | isoyzme SCO1725 shows 92% residual activity in the presence of 10 mM Mg2+; isozyme SCO7513 shows 83% residual activity in the presence of 10 mM Mg2+ | Streptomyces coelicolor | |
| additional information | Ca2+ and Mg2+ do not significantly reduce the lipolytic activity, resistant to inhibition by phenylmethylsulfonyl fluoride; Ca2+ and Mg2+ do not significantly reduce the lipolytic activity, resistant to inhibition by phenylmethylsulfonyl fluoride | Streptomyces coelicolor |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23980 | - |
SCO1725, estimated molecular weight | Streptomyces coelicolor |
| 26970 | - |
SCO7513, estimated molecular weight | Streptomyces coelicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | Q93J06 | - |
- |
| Streptomyces coelicolor | Q9S2A5 | - |
- |
| Streptomyces coelicolor A3(2) | Q93J06 | - |
- |
| Streptomyces coelicolor A3(2) | Q9S2A5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q Sepharose column chromatography, CM Sepharose FF column chromatography, and DEAE Sepharose column chromatography | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl caprate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl caprate + H2O | - |
Streptomyces coelicolor A3(2) | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Streptomyces coelicolor A3(2) | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Streptomyces coelicolor A3(2) | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl stearate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + stearate | - |
? | |
| 4-nitrophenyl stearate + H2O | - |
Streptomyces coelicolor A3(2) | 4-nitrophenol + stearate | - |
? | |
| additional information | isozyme SCO1725 shows no activity on tricaprin, tricaproin, and olive oil, 4-nitrophenyl stearate is hardly hydrolysed, 4-nitrophenyl stearate is no substrate for isozyme SCO1725 | Streptomyces coelicolor | ? | - |
? | |
| additional information | isozyme SCO1725 shows no activity on tricaprin, tricaproin, and olive oil, 4-nitrophenyl stearate is hardly hydrolysed, 4-nitrophenyl stearate is no substrate for isozyme SCO1725 | Streptomyces coelicolor A3(2) | ? | - |
? | |
| tributyrin + H2O | partially hydrolysed by isozyme SCO1725 | Streptomyces coelicolor | dibutyrin + butyrate | - |
? | |
| tributyrin + H2O | partially hydrolysed by isozyme SCO1725 | Streptomyces coelicolor A3(2) | dibutyrin + butyrate | - |
? | |
| tricaprin + H2O | - |
Streptomyces coelicolor | dicaprin + decaprate | - |
? | |
| tricaprylin + H2O | - |
Streptomyces coelicolor | dicaprylin + caprylate | - |
? | |
| tricaprylin + H2O | - |
Streptomyces coelicolor A3(2) | dicaprylin + caprylate | - |
? | |
| Tween 80 + H2O | - |
Streptomyces coelicolor | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Streptomyces coelicolor |
| SCO1725 | isozyme | Streptomyces coelicolor |
| SCO7513 | isozyme | Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | isozyme SCO1725 | Streptomyces coelicolor |
| 45 | 55 | isozyme SCO7513 | Streptomyces coelicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | isozyme SCO1725 is stable when incubated for 30 min at temperatures in the range of 30-70°C (at pH 7) | Streptomyces coelicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | isozyme SCO1725 | Streptomyces coelicolor |
| 8.5 | 10 | isozyme SCO7513 | Streptomyces coelicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 10 | 11 | the isozyme SCO1725 becomes unstable at pH over 10, where the activity drops to 34% at pH 10.5 and to 8% at pH 11 | Streptomyces coelicolor |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces coelicolor | isozyme SCO7513, predicted value | - |
4.73 |
| Streptomyces coelicolor | isozyme SCO1725, predicted value | - |
6.23 |
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