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Literature summary for 3.1.1.3 extracted from

  • Dutta, S.; Ray, L.
    Production and characterization of an alkaline thermostable crude lipase from an isolated strain of Bacillus cereus C(7) (2009), Appl. Biochem. Biotechnol., 159, 142-154.
    View publication on PubMed
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate 23% increase in activity at 0.5% (w/v) Bacillus cereus
sodium taurocholate 20% increase in activity at 0.5% (w/v) Bacillus cereus

General Stability

General Stability Organism
the enzyme remains stable in the presence of trypsin Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
benzalkonium chloride little inhibitory effect Bacillus cereus
Brij 35 little inhibitory effect Bacillus cereus
cetyltrimethylammonium bromide
-
 Bacillus cereus
cholic acid 90% residual activity at 0.5% (w/v) Bacillus cereus
Co2+ 38.83% residual activity at 5 mM Bacillus cereus
Fe3+ complete inhibition at 5 mM Bacillus cereus
Mn2+ complete inhibition at 5 mM Bacillus cereus
Polyethylene glycol little inhibitory effect Bacillus cereus
Sn2+ complete inhibition at 5 mM Bacillus cereus
Span 40 little inhibitory effect Bacillus cereus
Triton X-100
-
 Bacillus cereus
Tween 20 little inhibitory effect Bacillus cereus
Tween 80 little inhibitory effect Bacillus cereus
Zn2+ 72.41% residual activity at 5 mM Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 104% increase in activity in the presence of 1 mM Ca2+ Bacillus cereus
Co2+ 112.56% increase in activity in the presence of 0.1 mM Co2+, the stimulatory effect is not observed at higher concentrations Bacillus cereus
Cu2+ 83.03% increase in activity in the presence of 1 mM Cu2+ Bacillus cereus
Mg2+ 46.66% increase in activity in the presence of 0.1 mM Mg2+, the stimulatory effect is not observed at higher concentrations Bacillus cereus
Zn2+ 41.5% increase in activity in the presence of 1 mM Zn2+, the stimulatory effect is not observed at higher concentrations Bacillus cereus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information commercial detergents enhance the enzyme activity, the enzyme retains 60% or more activity in presence of commercial detergents Bacillus cereus
SDS SDS has little stimulatory effect Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus C(7)
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is stable in the presence of oxidizing agents like 1% (v/v) hydrogen peroxide and 1% (w/v) sodium hypochlorite Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
olive oil + H2O
-
 Bacillus cereus ?
-
 ?
olive oil + H2O
-
 Bacillus cereus C(7) ?
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 lipase activity increases progressively with increase in temperature and maximum activity is found to be at 60°C, then the activity decreases with further increase in temperature Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
7.6 9 at 60°C, using Tris-HCl buffer Bacillus cereus
9 10 at 60°C, using glycine-NaOH buffer Bacillus cereus