Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
SDS-PAGE | Acinetobacter sp. |
Organic Solvent | Comment | Organism |
---|---|---|
hydrogen peroxide | the enzyme shows stability in the presence of 30% (w/v) hydrogen peroxide | Acinetobacter sp. |
additional information | slight decrease in activity in the presence of commercial detergents | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. MTCC 6816 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified by by polyethylene glycol-salt aqueous two phase system and octyl Sepharose column chromatography | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.9 | - |
crude extract, in 0.1 M Tris buffer, pH 8.5 | Acinetobacter sp. |
88.8 | - |
after 14.92fold purification, in 0.1 M Tris buffer, pH 8.5 | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl laurate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Acinetobacter sp. MTCC 6816 | 4-nitrophenol + laurate | - |
? | |
olive oil + H2O | - |
Acinetobacter sp. | ? | - |
? | |
olive oil + H2O | - |
Acinetobacter sp. MTCC 6816 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Acinetobacter sp. |
triacylglycerol hydrolase | - |
Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Acinetobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | the lipase is active at low temperatures and retains 86.8% activity at 10°C, when the enzyme is incubated with 0.1 M Tris buffer, pH 8.5 for 1 h, it is stable up to 50°C | Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Acinetobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | - |
Acinetobacter sp. |