Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | - |
Bacillus cereus | |
Tween-20 | - |
Bacillus cereus | |
Tween-40 | - |
Bacillus cereus | |
Tween-80 | - |
Bacillus cereus |
Application | Comment | Organism |
---|---|---|
synthesis | the enantioselective lipase shows potential to be used as a catalyst to prepare optically pure pharmaceuticals | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Bacillus cereus | |
Cu2+ | - |
Bacillus cereus | |
K+ | - |
Bacillus cereus | |
Mg2+ | - |
Bacillus cereus | |
Mn2+ | - |
Bacillus cereus | |
Ni2+ | - |
Bacillus cereus | |
SDS | - |
Bacillus cereus | |
Zn2+ | - |
Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is not affected by Ca2+ or Na+ | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus C71 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from strain C71 1092fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, and two steps of ion exchange chromatography | Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
218.5 | - |
purified enzyme, substrate (R,S)-flurbiprofen ethyl ester | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-flurbiprofen ethyl ester + H2O | enantioselective reaction | Bacillus cereus | (S)-flurbiprofen ethyl ester + (R)-flurbiprofen + ethanol | - |
? | |
(R,S)-flurbiprofen ethyl ester + H2O | enantioselective reaction | Bacillus cereus C71 | (S)-flurbiprofen ethyl ester + (R)-flurbiprofen + ethanol | - |
? | |
ethyl 2-arylpropanoate + H2O | - |
Bacillus cereus | ethanol + (R)-2-arylpropanoate | - |
? | |
ethyl 2-arylpropanoate + H2O | - |
Bacillus cereus C71 | ethanol + (R)-2-arylpropanoate | - |
? | |
additional information | substrate specificity, the enantioselective lipase hydrolyzes 4-nitrophenyl esters with acyl chain lengths between C4 and C12, and shows high enantioselectivity toward the R-isomer of ethyl 2-arylpropanoate, overview | Bacillus cereus | ? | - |
? | |
additional information | substrate specificity, the enantioselective lipase hydrolyzes 4-nitrophenyl esters with acyl chain lengths between C4 and C12, and shows high enantioselectivity toward the R-isomer of ethyl 2-arylpropanoate, overview | Bacillus cereus C71 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
enantioselective lipase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
- |
Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 35 | 3 h, purified enzyme, 92% remaining activity at 35°C | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Bacillus cereus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | purified enzyme, stable | Bacillus cereus |