Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | activates both extracellular isozymes 1.73fold | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | no inhibition of extracellular isozyme 1 by EDTA up to 10 mM, but 80% inhibition of extracellular isozyme 2 by 10 mM EDTA | Pseudomonas aeruginosa | |
PMSF | inhibition of both extracellular isozymes | Pseudomonas aeruginosa | |
Zn2+ | activates extracellular isozyme 1 slightly, inhibits extracellular isozyme 2 | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | two isozymes | Pseudomonas aeruginosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | increases the thermostability and activity of both extracellular isozymes | Pseudomonas aeruginosa | |
Mg2+ | activates extracellular isozyme 2 slightly | Pseudomonas aeruginosa | |
additional information | the extracellular isozymes are not affected by SDS | Pseudomonas aeruginosa | |
Na+ | activates extracellular isozyme1 slightly | Pseudomonas aeruginosa | |
Zn2+ | activates extracellular isozyme 1 slightly, inhibits extracellular isozyme 2 | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15500 | - |
1 * 15500, extracellular isozyme 1, SDS-PAGE, 1 * 54970, extracellular isozyme 2, SDS-PAGE | Pseudomonas aeruginosa |
54970 | - |
1 * 15500, extracellular isozyme 1, SDS-PAGE, 1 * 54970, extracellular isozyme 2, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
two extracellular isozymes | - |
Pseudomonas aeruginosa Ps-x | - |
two extracellular isozymes | - |
Purification (Comment) | Organism |
---|---|
two native extracellular isozymes from strain Ps-x by ammonium sulfate fractionation, and anion and cation exchange chromatography, extracellular isozyme 1 is purified 6.36fold, and extracellular isozyme 2 is purified 14.76fold | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
165.5 | - |
purified extracellular isozyme 1 | Pseudomonas aeruginosa |
384 | - |
purified extracellular isozyme 2 | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa Ps-x | 4-nitrophenol + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 15500, extracellular isozyme 1, SDS-PAGE, 1 * 54970, extracellular isozyme 2, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
extracellular lipase | - |
Pseudomonas aeruginosa |
lipase I | - |
Pseudomonas aeruginosa |
lipase II | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
extracellular isozyme 1 | Pseudomonas aeruginosa |
50 | - |
extracellular isozyme 2 | Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Ca2+ increases the thermostability of both extracellular isozymes at 40-50°C | Pseudomonas aeruginosa |
70 | - |
60 min, extracellular isozyme 1 retains 47.7% activity, while extracellular isozyme 2 retains 36.7% activity | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
extracellular isozyme 2 | Pseudomonas aeruginosa |
10 | - |
extracellular isozyme 1 | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Pseudomonas aeruginosa |