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Literature summary for 3.1.1.3 extracted from

  • Saeed, H.M.; Zaghloul, T.I.; Khalil, A.I.; Abdelbaeth, M.T.
    Purification and characterization of two extracellular lipases from Pseudomonas aeruginosa Ps-x (2005), Pol. J. Microbiol., 54, 233-240.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DMSO activates both extracellular isozymes 1.73fold Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
EDTA no inhibition of extracellular isozyme 1 by EDTA up to 10 mM, but 80% inhibition of extracellular isozyme 2 by 10 mM EDTA Pseudomonas aeruginosa
PMSF inhibition of both extracellular isozymes Pseudomonas aeruginosa
Zn2+ activates extracellular isozyme 1 slightly, inhibits extracellular isozyme 2 Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular two isozymes Pseudomonas aeruginosa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ increases the thermostability and activity of both extracellular isozymes Pseudomonas aeruginosa
Mg2+ activates extracellular isozyme 2 slightly Pseudomonas aeruginosa
additional information the extracellular isozymes are not affected by SDS Pseudomonas aeruginosa
Na+ activates extracellular isozyme1 slightly Pseudomonas aeruginosa
Zn2+ activates extracellular isozyme 1 slightly, inhibits extracellular isozyme 2 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15500
-
1 * 15500, extracellular isozyme 1, SDS-PAGE, 1 * 54970, extracellular isozyme 2, SDS-PAGE Pseudomonas aeruginosa
54970
-
1 * 15500, extracellular isozyme 1, SDS-PAGE, 1 * 54970, extracellular isozyme 2, SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
two extracellular isozymes
-
Pseudomonas aeruginosa Ps-x
-
two extracellular isozymes
-

Purification (Commentary)

Purification (Comment) Organism
two native extracellular isozymes from strain Ps-x by ammonium sulfate fractionation, and anion and cation exchange chromatography, extracellular isozyme 1 is purified 6.36fold, and extracellular isozyme 2 is purified 14.76fold Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
165.5
-
purified extracellular isozyme 1 Pseudomonas aeruginosa
384
-
purified extracellular isozyme 2 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl palmitate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Pseudomonas aeruginosa Ps-x 4-nitrophenol + palmitate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 15500, extracellular isozyme 1, SDS-PAGE, 1 * 54970, extracellular isozyme 2, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
extracellular lipase
-
Pseudomonas aeruginosa
lipase I
-
Pseudomonas aeruginosa
lipase II
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
extracellular isozyme 1 Pseudomonas aeruginosa
50
-
extracellular isozyme 2 Pseudomonas aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 90
-
Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Ca2+ increases the thermostability of both extracellular isozymes at 40-50°C Pseudomonas aeruginosa
70
-
60 min, extracellular isozyme 1 retains 47.7% activity, while extracellular isozyme 2 retains 36.7% activity Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
extracellular isozyme 2 Pseudomonas aeruginosa
10
-
extracellular isozyme 1 Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Pseudomonas aeruginosa