Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activate slightly | Serratia marcescens | |
DTT | activate slightly | Serratia marcescens | |
additional information | effects of surfactants on the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester into (S)-flurbiprofen | Serratia marcescens | |
Triton X-207 | activates the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester | Serratia marcescens | |
Triton X-305 | activates the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester | Serratia marcescens |
Cloned (Comment) | Organism |
---|---|
phylogenetic tree | Serratia marcescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Serratia marcescens | |
EDTA | - |
Serratia marcescens | |
Mg2+ | slightly inhibits the reaction activity but enhances the enantioselectivity of the hydrolysis of (R,S)-flurbiprofen ethyl ester | Serratia marcescens | |
additional information | effects of surfactants on the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester into (S)-flurbiprofen, PMSF are poor inhibitors | Serratia marcescens | |
SDS | - |
Serratia marcescens | |
Zn2+ | - |
Serratia marcescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Serratia marcescens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester | Serratia marcescens | |
Co2+ | activates slightly, but reduces enantioslectivity | Serratia marcescens | |
Mn2+ | activates slightly, but reduces enantioslectivity | Serratia marcescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64900 | - |
x * 64900, SDS-PAGE | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Serratia marcescens ES-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 56fold from cell culture supernatant by ultrafiltration, hydrophobic interaction chromatography, and anion exchange chromatography | Serratia marcescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
118 | - |
purified native enzyme | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-flurbiprofen ethyl ester + H2O | enantioselective hydrolysis, kinetic resolution, overview | Serratia marcescens | (S)-flurbiprofen + ethanol | - |
? | |
(R,S)-flurbiprofen ethyl ester + H2O | enantioselective hydrolysis, kinetic resolution, overview | Serratia marcescens ES-2 | (S)-flurbiprofen + ethanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64900, SDS-PAGE | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
extracellular lipase | - |
Serratia marcescens |
triacylglycerol acylhydrolase | - |
Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Serratia marcescens |