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Literature summary for 3.1.1.3 extracted from

  • Bae, H.; Lee, K.; Lee, Y.
    Enantioselective properties of extracellular lipase from Serratia marcescens ES-2 for kinetic resolution of (S)-flurbiprofen (2006), J. Mol. Catal. B, 40, 24-29.
No PubMed abstract available
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activate slightly Serratia marcescens
DTT activate slightly Serratia marcescens
additional information effects of surfactants on the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester into (S)-flurbiprofen Serratia marcescens
Triton X-207 activates the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester Serratia marcescens
Triton X-305 activates the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester Serratia marcescens

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic tree Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Serratia marcescens
EDTA
-
 Serratia marcescens
Mg2+ slightly inhibits the reaction activity but enhances the enantioselectivity of the hydrolysis of (R,S)-flurbiprofen ethyl ester Serratia marcescens
additional information effects of surfactants on the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester into (S)-flurbiprofen, PMSF are poor inhibitors Serratia marcescens
SDS
-
 Serratia marcescens
Zn2+
-
 Serratia marcescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Serratia marcescens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates the enantioselective hydrolysis of (R,S)-flurbiprofen ethyl ester Serratia marcescens
Co2+ activates slightly, but reduces enantioslectivity Serratia marcescens
Mn2+ activates slightly, but reduces enantioslectivity Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64900
-
 x * 64900, SDS-PAGE Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-
Serratia marcescens ES-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 56fold from cell culture supernatant by ultrafiltration, hydrophobic interaction chromatography, and anion exchange chromatography Serratia marcescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
118
-
 purified native enzyme Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-flurbiprofen ethyl ester + H2O enantioselective hydrolysis, kinetic resolution, overview Serratia marcescens (S)-flurbiprofen + ethanol
-
 ?
(R,S)-flurbiprofen ethyl ester + H2O enantioselective hydrolysis, kinetic resolution, overview Serratia marcescens ES-2 (S)-flurbiprofen + ethanol
-
 ?

Subunits

Subunits Comment Organism
? x * 64900, SDS-PAGE Serratia marcescens

Synonyms

Synonyms Comment Organism
extracellular lipase
-
 Serratia marcescens
triacylglycerol acylhydrolase
-
 Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Serratia marcescens