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Literature summary for 3.1.1.3 extracted from
- Molecular cloning and functional expression of esf gene encoding enantioselective lipase from Serratia marcescens ES-2 for kinetic resolution of optically active (S)-flurbiprofen (2007), J. Microbiol. Biotechnol., 17, 74-80.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Triton X-207 induces and enhances enzyme secretion from the overexpressing Serratia marcescens strain best at 3.0%, effect of surfactants on secretion of enantioselective Esf lipase in transformant strain BESF overexpressing the esf gene | Serratia marcescens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene esf, construction and screening of a genetic library, DNA and amino acid sequence determination and analysis, reintroduction, overexpression in, and secretion from the transformed parent strain ES-2, i.e. strain BESF, expression as His-tagged enzyme in Escherichia coli | Serratia marcescens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | enzyme secretion, the Esf lipase does not possess an N-terminal signal sequence commonly required for the secretion of lipase, but it contains an extreme C-terminal motif consisting of a hydrophobic five-residue sequence that can be recognized by an ABC-transporter system correlated to a signal peptide-independent mechanism for the secretion of protein in Gram-negative bacteria | Serratia marcescens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates 4.6fold at 10 mM, the Esf lipase contains a nine-residue GGXGXDXXX sequence motif in the upstream region of the C-terminal motif, known to form a parallel beta-roll structure binding Ca2+ ions | Serratia marcescens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64978 | - |
x * 64978, sequence calculation, x * 65000, wild-type enzyme, SDS-PAGE, x * 69000, recombinant His-tagged enzyme | Serratia marcescens |
| 65000 | - |
x * 64978, sequence calculation, x * 65000, wild-type enzyme, SDS-PAGE, x * 69000, recombinant His-tagged enzyme | Serratia marcescens |
| 69000 | - |
x * 64978, sequence calculation, x * 65000, wild-type enzyme, SDS-PAGE, x * 69000, recombinant His-tagged enzyme | Serratia marcescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia marcescens | Q0MVP2 | gene esf | - |
| Serratia marcescens ES-2 | Q0MVP2 | gene esf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native secreted enantioselective lipase 12.1fold from culture supernatant by ultrafiltration, hydrophobic interaction and anion exchange chromatography | Serratia marcescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Serratia marcescens |
| 58.4 | - |
purified Esf lipase | Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R,S)-flurbiprofen ethyl ester + H2O | high enantiomeric excess and conversion yield of 98% and 48%, respectively | Serratia marcescens | (S)-flurbiprofen + ethanol | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Serratia marcescens | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Serratia marcescens ES-2 | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caprate + H2O | high activity | Serratia marcescens | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl caproate + H2O | high activity | Serratia marcescens | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl caproate + H2O | high activity | Serratia marcescens ES-2 | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl caprylate + H2O | best 4-nitrophenyl ester substrate | Serratia marcescens | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl caprylate + H2O | best 4-nitrophenyl ester substrate | Serratia marcescens ES-2 | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Serratia marcescens | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl myristate + H2O | - |
Serratia marcescens | 4-nitrophenol + myristate | - |
? | |
| 4-nitrophenyl palmitate + H2O | low activity | Serratia marcescens | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl propionate + H2O | low activity | Serratia marcescens | 4-nitrophenol + propionate | - |
? | |
| 4-nitrophenyl propionate + H2O | low activity | Serratia marcescens ES-2 | 4-nitrophenol + propionate | - |
? | |
| additional information | substrate specificity, no or poor activity with tributyrin, triacetin, tripropionin, tristearin, 4-nitrophenyl stearate, and 4-nitrophenyl acetate, the lipase shows preferential substrate specificity toward the medium-chain-length fatty acids, overview | Serratia marcescens | ? | - |
? | |
| additional information | substrate specificity, no or poor activity with tributyrin, triacetin, tripropionin, tristearin, 4-nitrophenyl stearate, and 4-nitrophenyl acetate, the lipase shows preferential substrate specificity toward the medium-chain-length fatty acids, overview | Serratia marcescens ES-2 | ? | - |
? | |
| tricaprin + H2O | best triacylglyceride substrate | Serratia marcescens | dicaprin + caprate | - |
? | |
| tricaproin + H2O | - |
Serratia marcescens | dicaproin + caproate | - |
? | |
| tricaprylin + H2O | - |
Serratia marcescens | dicaprylin + caprylate | - |
? | |
| trilaurin + H2O | high activity | Serratia marcescens | dilaurin + laurate | - |
? | |
| trimyristin + H2O | - |
Serratia marcescens | dimyristin + myristate | - |
? | |
| tripalmitin + H2O | low activity | Serratia marcescens | dipalmitin + palmitate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64978, sequence calculation, x * 65000, wild-type enzyme, SDS-PAGE, x * 69000, recombinant His-tagged enzyme | Serratia marcescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enantioselective lipase | - |
Serratia marcescens |
| Esf lipase | - |
Serratia marcescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Serratia marcescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | - |
Serratia marcescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Serratia marcescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | - |
Serratia marcescens |
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