Literature summary for 3.1.1.3 extracted from

Griffon, N.; Budreck, E.C.; Long, C.J.; Broedl, U.C.; Marchadier, D.H.; Glick, J.M.; Rader, D.J.
Substrate specificity of lipoprotein lipase and endothelial lipase: studies of lid chimeras (2006), J. Lipid Res., 47, 1803-1811.

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Protein Variants

Protein Variants	Comment	Organism
E250Q	site-directed mutagenesis, the mutant shows reduced triacylglyceride lipase activity compared to the wild-type endothelial lipase	Homo sapiens
G241R	site-directed mutagenesis, the mutant shows reduced triacylglyceride lipase activity compared to the wild-type endothelial lipase	Homo sapiens
additional information	construction of an 245R insertion mutant showing increased triacylglyceride lipase activity compared to the wild-type endothelial lipase, exchange of lid regions of the endothelial lipase with the one of the lipoprotein lipase, EC 3.1.1.34, resulting in a substantial increase of 4.2fold in the ratio of triglyceride lipase activity to phospholipase activity compared with wild-type endothelial lipase, but did not fully confer the high degree of preference of lipoprotein lipase for triacylglyceride substrates, the replacement of the lipoprotein lipase lid by the endothelial lipase lid consistently generated a decrease in the ratio of triglyceride lipase activity to phospholipase activity to 32.7% of the ratio of wild-type lipoprotein lipase, overview, domains tructure of chimeric proteins, overview	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
triacylglycerol + H2O	Homo sapiens	endothelial lipase plays a central role in plasma lipoprotein metabolism	diacylglycerol + a carboxylate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activities of wild-type and mutant enzymes, overview	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
triacylglycerol + H2O	endothelial lipase plays a central role in plasma lipoprotein metabolism	Homo sapiens	diacylglycerol + a carboxylate	-	?
triacylglycerol + H2O	the endothelial lipase is more active as a phospholipase than as a triacylglyceride lipase	Homo sapiens	diacylglycerol + a carboxylate	-	?
triolein + H2O	-	Homo sapiens	diolein + oleate	-	?

Synonyms

Synonyms	Comment	Organism
endothelial lipase	-	Homo sapiens
More	the enzyme belongs to the triglyceride lipase gene subfamily	Homo sapiens
triglyceride lipase	-	Homo sapiens

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Release 2023.1 (January 2023)