Application | Comment | Organism |
---|---|---|
synthesis | application of the immobilized lipase in non-conventional biocatalysis for the synthesis of surfactants and biodiesel, overview | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the pancreatic lipase on a polysiloxane-polyvinyl alcohol hybrid matrix with 250 U/g matrix, the immobilized enzyme shows high activity converting triglycerides to fatty acid alkyl esters attaining yields varying from 75% to 95% | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
330 | - |
olive oil | pH 7.0, 37°C, immobilized lipase | Sus scrofa | |
534 | - |
olive oil | pH 7.0, 37°C, free lipase | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P00591 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | from pancreas | Sus scrofa | - |
pancreas | commercial preparation | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
olive oil + H2O | - |
Sus scrofa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pancreatic lipase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
free lipase | Sus scrofa |
45 | - |
immobilized lipase | Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | activity range of free lipase and immobilized lipase, over 80% of maximal activity at 35°C-55°C of the immobilized lipase | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
1 h, the free lipase shows 20% remaining activity | Sus scrofa |
50 | - |
1 h, complete inactivation of the free lipase | Sus scrofa |
60 | - |
1 h, the immobilized lipase shows 14.3% remaining activity | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
free lipase and immobilized lipase | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the immobilization procedure broadened the pH working range of the free lipase | Sus scrofa |
7 | 8.5 | over 80% of maximal activity within this range, immobilized lipase | Sus scrofa |