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Literature summary for 3.1.1.3 extracted from

  • Sekhon, A.; Dahiya, N.; Tiwari, R.P.; Hoondal, G.S.
    Properties of a thermostable extracellular lipase from Bacillus megaterium AKG-1 (2005), J. Basic Microbiol., 45, 147-154.
    View publication on PubMed
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
acetone activates 20-70% at 20% v/v Priestia megaterium
Brij 52 23.6% activation at 0.1% w/v Priestia megaterium
cholic acid 13% activation at 0.1% w/v Priestia megaterium
deoxycholic acid 76% activation at 0.1% w/v Priestia megaterium
DMSO activates 20-70% at 20% v/v Priestia megaterium
Isopropanol activates 20-70% at 10% v/v Priestia megaterium
lithocholic acid 24% activation at 0.1% w/v Priestia megaterium
rhamnolipid 24% activation at 0.1% w/v Priestia megaterium
sodium deoxycholate 31% activation at 0.1% w/v Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 20% inhibition Priestia megaterium
Cu2+ strong inhibition Priestia megaterium
Hg2+ strong inhibition Priestia megaterium
Ni2+ 20% inhibition Priestia megaterium
Pb2+ 20% inhibition Priestia megaterium
SDS 42% inhibition at 0.1% w/v Priestia megaterium
Zn2+ strong inhibition Priestia megaterium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Priestia megaterium
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effects by Ca2+, K+ and Li+ Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
 isolated from soil samples collected from Kargil region
-
Priestia megaterium AKG-1
-
 isolated from soil samples collected from Kargil region
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 5.3fold by ammonium sulfate fractionation Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
414
-
 partially purified enzyme Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-acetoxy beta-lactam + H2O stereospecificity in hydrolysis of 3-acetoxy beta-lactam Priestia megaterium ?
-
 ?
3-acetoxy beta-lactam + H2O stereospecificity in hydrolysis of 3-acetoxy beta-lactam Priestia megaterium AKG-1 ?
-
 ?
4-nitrophenyl laurate + H2O
-
 Priestia megaterium 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O
-
 Priestia megaterium AKG-1 4-nitrophenol + laurate
-
 ?
tributyrin + H2O
-
 Priestia megaterium dibutyrin + butyrate
-
 ?
tributyrin + H2O
-
 Priestia megaterium AKG-1 dibutyrin + butyrate
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Priestia megaterium

Synonyms

Synonyms Comment Organism
lipase
-
 Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Priestia megaterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90 temperature profile Priestia megaterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, completely stable Priestia megaterium
70
-
 30 min, 50% remaining activity Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Priestia megaterium

pH Range

pH Minimum pH Maximum Comment Organism
5 10.5 pH profile Priestia megaterium