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Literature summary for 3.1.1.3 extracted from

  • Ogino, H.; Katou, Y.; Akagi, R.; Mimitsuka, T.; Hiroshima, S.; Gemba, Y.; Doukyu, N.; Yasuda, M.; Ishimi, K.; Ishikawa, H.
    Cloning and expression of gene, and activation of an organic solvent-stable lipase from Pseudomonas aeruginosa LST-03 (2007), Extremophiles, 11, 809-817.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lip9, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) inclusion bodies, subcloning in Escherichia coli strain JM109 Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular LST-03 lipase is secreted Pseudomonas aeruginosa
-
-

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa A8QYB2 LST-03 lipase; gene lip9
-
Pseudomonas aeruginosa LST-03 A8QYB2 LST-03 lipase; gene lip9
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, after solubilization from inclusion bodies, by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration to homogeneity Pseudomonas aeruginosa

Renatured (Commentary)

Renatured (Comment) Organism
recombinant enzyme from Escherichia coli inclusion bodies by 20 mM Tris-HCl buffer, pH 8.0, and 5.3 M urea, followed by a 100fold dilution Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dimercaptopropan-1-ol tributyrate + H2O liberation of thiol groups by hydrolysis Pseudomonas aeruginosa ?
-
?
2,3-dimercaptopropan-1-ol tributyrate + H2O liberation of thiol groups by hydrolysis Pseudomonas aeruginosa LST-03 ?
-
?

Synonyms

Synonyms Comment Organism
Lip9
-
Pseudomonas aeruginosa
LST-03 lipase
-
Pseudomonas aeruginosa
organic solvent-stable lipase
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas aeruginosa