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Literature summary for 3.1.1.3 extracted from
- Influence of the functional activating agent on the biochemical and kinetic properties of Candida rugosa lipase immobilized on chemically modified cellulignin (2007), Biochem. Eng. J., 34, 13-19.
No PubMed abstract available
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the enzyme is immobilized on chemically modified wood cellulignin from Eucaliptus grandis by covalent binding using different activating agents such as carbonyldiimidazole, glutaraldehyde and sodium metaperiodate, method development and immobilization reaction mechanisms, overview | Diutina rugosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Diutina rugosa | |
| 0.136 | - |
4-nitrophenyl palmitate | pH 7.0, 37°C, enzyme immobilized with glutaraldehyde | Diutina rugosa |  |
| 0.158 | - |
4-nitrophenyl palmitate | pH 7.0, 37°C, enzyme immobilized with sodium metaperiodate | Diutina rugosa | |
| 0.17 | - |
4-nitrophenyl palmitate | pH 7.0, 37°C, free enzyme | Diutina rugosa | |
| 0.274 | - |
4-nitrophenyl palmitate | pH 7.0, 37°C, enzyme immobilized with carbonyldiimidazole | Diutina rugosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Diutina rugosa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Diutina rugosa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of immobilized enzyme using different immobilization agents, overview | Diutina rugosa |
| 974 | - |
commercially available purified enzyme preparation | Diutina rugosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | - |
Diutina rugosa | 4-nitrophenol + palmitate | - |
? | |
| olive oil + H2O | - |
Diutina rugosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Diutina rugosa |
| type VII lipase | - |
Diutina rugosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
free enzyme | Diutina rugosa |
| 40 | 50 | immobilized enzyme dependent on the immobilization agent, overview | Diutina rugosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
free enzyme | Diutina rugosa |
| 7 | 8.5 | immobilized enzyme dependent on the immobilization agent, overview | Diutina rugosa |
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