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Literature summary for 3.1.1.3 extracted from
- A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification (2006), Appl. Microbiol. Biotechnol., 73, 349-355.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | LipB68 acts as biocatalysts in biodiesel production and produces biodiesel with a yield of 92% after 12 h, at the lowest temperature of 20°C, catalyzing the transesterification reaction of biodiesel production, thus LipB68 represents a highly competitive energy-saving biocatalyst | Pseudomonas fluorescens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lipB68, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21(DE3) inclusion bodies | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | Q670W0 | isolated from soil samples of China, gene lipB68 | - |
| Pseudomonas fluorescens B68 | Q670W0 | isolated from soil samples of China, gene lipB68 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant LipB68 protein from Escherichia coli strain BL21(DE3) after solubilization from inclusion bodies, to 95% purity | Pseudomonas fluorescens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant LipB68 from Escherichia coli inclusion bodies by 8 M urea and subsequent dialysis, 25 mM Tris-HCl, pH 8.0 | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl caprate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + caprate | - |
? |  |
| 4-nitrophenyl caprate + H2O | - |
Pseudomonas fluorescens B68 | 4-nitrophenol + caprate | - |
? | |
| additional information | LipB68 performs the enantioselective acylation of racemic alcohols, e.g. toluene, and effectively catalyzes the transesterification of both alpha-phenylethanol and alpha-phenylpropanol at 20°C with high enantioselectivity, overview | Pseudomonas fluorescens | ? | - |
? | |
| additional information | LipB68 performs the enantioselective acylation of racemic alcohols, e.g. toluene, and effectively catalyzes the transesterification of both alpha-phenylethanol and alpha-phenylpropanol at 20°C with high enantioselectivity, overview | Pseudomonas fluorescens B68 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LipB68 | - |
Pseudomonas fluorescens |
| More | the enzyme is a member of lipase subfamily I.3 | Pseudomonas fluorescens |
| psychrophilic lipase | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Pseudomonas fluorescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 50 | temperature profile, overview | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudomonas fluorescens |
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