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Literature summary for 3.1.1.3 extracted from

  • Hasan, F.; Shah, A.A.; Hameed, A.
    Purification and characterization of a mesophilic lipase from Bacillus subtilis FH5 stable at high temperature and pH (2007), Acta Biol. Hung., 58, 115-132.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acetone inhibitory effect of organic solvents, overview Bacillus subtilis
Co2+
-
Bacillus subtilis
Cu2+
-
Bacillus subtilis
EDTA about 22% inhibition at 1 mM Bacillus subtilis
ethanol inhibitory effect of organic solvents, overview Bacillus subtilis
Isopropanol inhibitory effect of organic solvents, overview Bacillus subtilis
Na+
-
Bacillus subtilis
PMSF about 15% inhibition at 1 mM Bacillus subtilis
SDS 20% inhibition at 1 mM Bacillus subtilis
Zn2+
-
Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.05
-
4-nitrophenyl laurate pH 8.0, 37°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ about 7.5% activation at 1 mM Bacillus subtilis
Ca2+ 35% activation at 1 mM Bacillus subtilis
Fe2+ 35% activation at 1 mM Bacillus subtilis
Mg2+ 40% activation at 1 mM Bacillus subtilis
Mn2+ 35% activation at 1 mM Bacillus subtilis
additional information the enzyme is not affected by 1 mM of K+ Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
x * 62000, SDS-PAGE x * 24000, SDS-PAGE Bacillus subtilis
62000
-
x * 62000, SDS-PAGE x * 24000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
isolated from tannery wastes
-
Bacillus subtilis FH5
-
isolated from tannery wastes
-

Purification (Commentary)

Purification (Comment) Organism
native isozymes to homogeneity by acetone fractionation, anion exchange chromatography, gel filtration, and hydroxylapatite adsorption chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O
-
Bacillus subtilis 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O
-
Bacillus subtilis FH5 4-nitrophenol + laurate
-
?
long-chain triacylglycerol + H2O
-
Bacillus subtilis diacylglycerol + a carboxylate
-
?
long-chain triacylglycerol + H2O
-
Bacillus subtilis FH5 diacylglycerol + a carboxylate
-
?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE x * 24000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
mesophilic lipase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 temperature profile, overview Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
1 h, purified native enzyme, 99% remaining activity Bacillus subtilis
50
-
1 h, purified native enzyme, 92% remaining activity Bacillus subtilis
60
-
1 h, purified native enzyme, 76% remaining activity Bacillus subtilis
70
-
1 h, purified native enzyme, 38% remaining activity Bacillus subtilis
75
-
1 h, purified native enzyme, 25% remaining activity Bacillus subtilis
85
-
1 h, purified native enzyme, 9.7% remaining activity Bacillus subtilis
90
-
1 h, purified native enzyme, 6.9% remaining activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
4 11 pH profile, overview Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
1 h, 37°C, purified native enzyme, 14% remaining activity Bacillus subtilis
5
-
1 h, 37°C, purified native enzyme, 24% remaining activity Bacillus subtilis
6
-
1 h, 37°C, purified native enzyme, 44% remaining activity Bacillus subtilis
7
-
1 h, 37°C, purified native enzyme, 52% remaining activity Bacillus subtilis
8
-
1 h, 37°C, purified native enzyme, 68% remaining activity Bacillus subtilis
9
-
1 h, 37°C, purified native enzyme, 74% remaining activity Bacillus subtilis
10
-
1 h, 37°C, purified native enzyme, completely stable Bacillus subtilis
11
-
1 h, 37°C, purified native enzyme, 90% remaining activity Bacillus subtilis