Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | inhibitory effect of organic solvents, overview | Bacillus subtilis | |
Co2+ | - |
Bacillus subtilis | |
Cu2+ | - |
Bacillus subtilis | |
EDTA | about 22% inhibition at 1 mM | Bacillus subtilis | |
ethanol | inhibitory effect of organic solvents, overview | Bacillus subtilis | |
Isopropanol | inhibitory effect of organic solvents, overview | Bacillus subtilis | |
Na+ | - |
Bacillus subtilis | |
PMSF | about 15% inhibition at 1 mM | Bacillus subtilis | |
SDS | 20% inhibition at 1 mM | Bacillus subtilis | |
Zn2+ | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.05 | - |
4-nitrophenyl laurate | pH 8.0, 37°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 7.5% activation at 1 mM | Bacillus subtilis | |
Ca2+ | 35% activation at 1 mM | Bacillus subtilis | |
Fe2+ | 35% activation at 1 mM | Bacillus subtilis | |
Mg2+ | 40% activation at 1 mM | Bacillus subtilis | |
Mn2+ | 35% activation at 1 mM | Bacillus subtilis | |
additional information | the enzyme is not affected by 1 mM of K+ | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 62000, SDS-PAGE x * 24000, SDS-PAGE | Bacillus subtilis |
62000 | - |
x * 62000, SDS-PAGE x * 24000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
isolated from tannery wastes | - |
Bacillus subtilis FH5 | - |
isolated from tannery wastes | - |
Purification (Comment) | Organism |
---|---|
native isozymes to homogeneity by acetone fractionation, anion exchange chromatography, gel filtration, and hydroxylapatite adsorption chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl laurate + H2O | - |
Bacillus subtilis | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Bacillus subtilis FH5 | 4-nitrophenol + laurate | - |
? | |
long-chain triacylglycerol + H2O | - |
Bacillus subtilis | diacylglycerol + a carboxylate | - |
? | |
long-chain triacylglycerol + H2O | - |
Bacillus subtilis FH5 | diacylglycerol + a carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, SDS-PAGE x * 24000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
mesophilic lipase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | temperature profile, overview | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, purified native enzyme, 99% remaining activity | Bacillus subtilis |
50 | - |
1 h, purified native enzyme, 92% remaining activity | Bacillus subtilis |
60 | - |
1 h, purified native enzyme, 76% remaining activity | Bacillus subtilis |
70 | - |
1 h, purified native enzyme, 38% remaining activity | Bacillus subtilis |
75 | - |
1 h, purified native enzyme, 25% remaining activity | Bacillus subtilis |
85 | - |
1 h, purified native enzyme, 9.7% remaining activity | Bacillus subtilis |
90 | - |
1 h, purified native enzyme, 6.9% remaining activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | pH profile, overview | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
1 h, 37°C, purified native enzyme, 14% remaining activity | Bacillus subtilis |
5 | - |
1 h, 37°C, purified native enzyme, 24% remaining activity | Bacillus subtilis |
6 | - |
1 h, 37°C, purified native enzyme, 44% remaining activity | Bacillus subtilis |
7 | - |
1 h, 37°C, purified native enzyme, 52% remaining activity | Bacillus subtilis |
8 | - |
1 h, 37°C, purified native enzyme, 68% remaining activity | Bacillus subtilis |
9 | - |
1 h, 37°C, purified native enzyme, 74% remaining activity | Bacillus subtilis |
10 | - |
1 h, 37°C, purified native enzyme, completely stable | Bacillus subtilis |
11 | - |
1 h, 37°C, purified native enzyme, 90% remaining activity | Bacillus subtilis |