Cloned (Comment) | Organism |
---|---|
gene lip3, shotgun cloning, construction of a genomic library, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Pseudomonas aeruginosa |
General Stability | Organism |
---|---|
purified recombinant enzyme, 30°C, pH 8.0, half-life is 12.3 days | Pseudomonas aeruginosa |
Organic Solvent | Comment | Organism |
---|---|---|
1,4-butanediol | half-life is 7.4 days | Pseudomonas aeruginosa |
1,5-pentanediol | half-life is 0.19 days | Pseudomonas aeruginosa |
cyclohexane | half-life is 0.48 days | Pseudomonas aeruginosa |
dimethyl sulfoxide | half-life is 3.4 days | Pseudomonas aeruginosa |
Ethanol | half-life is 0.20 days | Pseudomonas aeruginosa |
Ethylene glycol | half-life is 84.6 days | Pseudomonas aeruginosa |
Glycerol | half-life is 37 days | Pseudomonas aeruginosa |
Methanol | half-life is 0.29 days | Pseudomonas aeruginosa |
additional information | strain LST-03 is organic solvent-tolerant, overview | Pseudomonas aeruginosa |
N,N-dimethylformamide | half-life is 7.9 days | Pseudomonas aeruginosa |
n-decane | half-life is 0.40 days | Pseudomonas aeruginosa |
n-heptane | half-life is 7.9 days | Pseudomonas aeruginosa |
n-hexane | half-life is 7.7 days | Pseudomonas aeruginosa |
n-octane | half-life is 9.2 days | Pseudomonas aeruginosa |
tert-Butanol | half-life is 0.17 days | Pseudomonas aeruginosa |
toluene | half-life is 0.37 days | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9KJG6 | gene lip3 | - |
Pseudomonas aeruginosa LST-03 | Q9KJG6 | gene lip3 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain JM109 by acetone fractionation, ion exchange and hydrophobic interaction chromatography to homogeneity | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
purified recombinant enzyme, 30°C, pH 8.0, half-life is 12.3 days | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dimercaptopropan-1-ol tributyrate + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
2,3-dimercaptopropan-1-ol tributyrate + H2O | - |
Pseudomonas aeruginosa LST-03 | ? | - |
? | |
tributyrin + H2O | - |
Pseudomonas aeruginosa | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Pseudomonas aeruginosa LST-03 | dibutyrin + butyrate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | almost no activity above 50°C | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable up to | Pseudomonas aeruginosa |
45 | - |
inactivation above | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | - |
Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | stable | Pseudomonas aeruginosa |