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Literature summary for 3.1.1.3 extracted from

  • Gilham, D.; Alam, M.; Gao, W.; Vance, D.E.; Lehner, R.
    Triacylglycerol hydrolase is localized to the endoplasmic reticulum by an unusual retrieval sequence where it participates in VLDL assembly without utilizing VLDL lipids as substrates (2005), Mol. Biol. Cell, 16, 984-996.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of wild-type and C-terminally truncated or non-glycosylated secreted mutant enzymes in RH-17777 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the truncated mutant enzyme shows altered localization and is not found, in contrary to the wild-type enzyme, in regions containing neutral lipid droplets, overview Homo sapiens
N79Q site-directed mutagenesis, disruption of N-glycosylation site, the N-glycosylation at Asn70 is not required for activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum in cisternae in proximity to mitochondria, the enzyme is targeted by an unusual retrieval sequence where it participates in VLDL assembly without utilizing VLDL lipids as substrates Homo sapiens 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme participates in VLDL assembly in endoplasmic reticulum without utilizing VLDL lipids as substrates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation at Asn70 is not required for activity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
H460M cell i.e. RH-17777 cell, large cell lung carcinoma cell line, low enzyme activity Homo sapiens
-
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O
-
 Homo sapiens 4-nitrophenol + laurate
-
 ?
additional information the enzyme participates in VLDL assembly in endoplasmic reticulum without utilizing VLDL lipids as substrates Homo sapiens ?
-
 ?
additional information substrate specificity of recombinant wild-type and mutant enzymes, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
TGH
-
 Homo sapiens
triacylglycerol hydrolase
-
 Homo sapiens