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Literature summary for 3.1.1.3 extracted from
- Protein engineering and applications of Candida rugosa lipase isoforms (2004), Lipids, 39, 513-526.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | the enzyme can be used for hydrolysis and synthesis of various esters, mutagenic modification and optimization of Candida rugosa isozymes for enantioselective, substrate-specific biocatalysis, improvement of thermostability, enantioselectivity, and substrate specificity, possible reactions are hydrolysis, direct esterification, acidolysis, alcoholysis, ester-interchange, and glycerolysis, overview | Diutina rugosa |
| nutrition | the enzyme of nonpathogenic Candida rugosa can be used for hydrolysis and synthesis of various esters in food application, mutagenic modification and optimization of isozymes for enantioselective, substrate-specific biocatalysis, improvement of thermostability, enantioselectivity, and substrate specificity, overview | Diutina rugosa |
| synthesis | the enzyme can be used for hydrolysis and synthesis of various esters, mutagenic modification and optimization of Candida rugosa isozymes for enantioselective, substrate-specific biocatalysis, improvement of thermostability, enantioselectivity, and substrate specificity, possible reactions are hydrolysis, direct esterification, acidolysis, alcoholysis, ester-interchange, and glycerolysis, overview | Diutina rugosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of LIP gene encoded wild-type and mutant isozymes in Saccharomyces cerevisiae and Pichia pastoris | Diutina rugosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | conversion by site-directed mutagenesis of most leucine triplets to serine, because the yeast cells do not use universal codons for leucine coding, which hinders improvement of isozymes for specific biocatalysis, overview | Diutina rugosa |
| N314Q | site-sirected mutagenesis, the mutation of the glycosylation site destabilizes the enzyme and affects the enzyme activity | Diutina rugosa |
| N351Q | site-sirected mutagenesis, the mutation of the glycosylation site destabilizes the enzyme and affects the enzyme activity | Diutina rugosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Diutina rugosa | enzyme regulation | ? | - |
? |  |
| tributyrin + H2O | Diutina rugosa | - |
dibutyrin + butyrate | - |
r | |
| triolein + H2O | Diutina rugosa | - |
diolein + oleate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Diutina rugosa | - |
formerly Candida cylindracea, several LIP genes, several isozymes | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | isozymes are glycoproteins, major glycosylation site is Asn351, other sites are Asn291 and Asn314, dependent on the isozyme, overview | Diutina rugosa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification isozymes from commercial preparations | Diutina rugosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad is formed by Ser209, Glu341, and His449, structure-function relationship and reaction mechanism, substrate binding, activity occurs via an interfacial activation process, a water-lipid interface is required for activity | Diutina rugosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | contains several isozymes | Diutina rugosa | - |
| additional information | expression analysis of different isozymes | Diutina rugosa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-O-dilauryl-rac-glycero-3-glutaric resorufin ester + H2O | commercial chromogenic lipase substrate | Diutina rugosa | ? | - |
? | |
| additional information | enzyme regulation | Diutina rugosa | ? | - |
? | |
| additional information | substrate specificities of wild-type and mutant isozymes, possible reactions are hydrolysis, direct esterification, acidolysis, alcoholysis, ester-interchange, and glycerolysis | Diutina rugosa | ? | - |
? | |
| tributyrin + H2O | - |
Diutina rugosa | dibutyrin + butyrate | - |
r | |
| triolein + H2O | - |
Diutina rugosa | diolein + oleate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | three-diemensional structure analysis, structure-function relationship, substrate binding | Diutina rugosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LIP | - |
Diutina rugosa |
| lipase | - |
Diutina rugosa |
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