General Stability | Organism |
---|---|
the purified enzyme is very stable to freezing and thawing | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | weak inhibition | Pseudomonas aeruginosa | |
additional information | no inhibition by EDTA at 1 mM | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme tends to form aggregates | Pseudomonas aeruginosa |
29000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa EF2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from medium of a Tween 80-limited culture, by anion exchange chromatography and gel filtration | Pseudomonas aeruginosa |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also shows 8fold lower esterase activity with 4-nitrophenyl acetate and Tween 80 as substrates | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme also shows 8fold lower esterase activity with 4-nitrophenyl acetate and Tween 80 as substrates | Pseudomonas aeruginosa EF2 | ? | - |
? | |
triolein + H2O | olive oil with predominantly triolein, regiospecificity for the 1- and 3-oleoyl residues | Pseudomonas aeruginosa | diolein + oleate | - |
? | |
triolein + H2O | olive oil with predominantly triolein, regiospecificity for the 1- and 3-oleoyl residues | Pseudomonas aeruginosa EF2 | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
extracellular lipase | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life is 17.5 min | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3000 | - |
triolein | - |
Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
4.9 |