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Literature summary for 3.1.1.3 extracted from
- Cloning and characterization of the acid lipase from castor beans (2004), J. Biol. Chem., 279, 45540-45545.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by NaCl | Ricinus communis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Ricinus communis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | - |
Ricinus communis |  |
| diethyl 4-nitrophenyl phosphate | - |
Ricinus communis | |
| EDTA | slight inhibition | Ricinus communis | |
| HgCl2 | - |
Ricinus communis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| oil body | associated to the surface of the oil body membrane in endosperm | Ricinus communis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight activation | Ricinus communis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, about, SDS-PAGE | Ricinus communis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Ricinus communis | the enzyme is involved in regulation of lipolysis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | Q5VKJ7 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| triacylglycerol + H2O = diacylglycerol + a carboxylate | the enzyme contains an active site serine residue | Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | restricted to | Ricinus communis | - |
| additional information | no expression in mature leaf, stem, and root, the enzyme expression decreases during germination, developmental and tissue-specific distribution | Ricinus communis | - |
| seed | - |
Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0034 | - |
purified recombinant enzyme, substrate triplamitin | Ricinus communis |
| 0.0054 | - |
purified recombinant enzyme, substrate triolein | Ricinus communis |
| 0.0058 | - |
purified recombinant enzyme, substrate trilinolein | Ricinus communis |
| 0.0073 | - |
purified recombinant enzyme, substrate triricinolein | Ricinus communis |
| 0.0089 | - |
purified recombinant enzyme, substrate trilaurin | Ricinus communis |
| 0.0214 | - |
purified recombinant enzyme, substrate tricaprin | Ricinus communis |
| 0.0242 | - |
purified recombinant enzyme, substrate 4-nitrophenyl butyrate | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | high activity | Ricinus communis | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Ricinus communis | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl palmitate + H2O | low activity | Ricinus communis | 4-nitrophenol + palmitate | - |
? | |
| additional information | the enzyme is involved in regulation of lipolysis | Ricinus communis | ? | - |
? | |
| additional information | substrate specificity, no activity with oleoyl-CoA and 1,2-dioleoyl-3-phosphatidylcholine | Ricinus communis | ? | - |
? | |
| tricaprin + H2O | - |
Ricinus communis | dicaprin + caprate | - |
? | |
| trilaurin + H2O | - |
Ricinus communis | dilaurin + laurate | - |
? | |
| trilinolein + H2O | - |
Ricinus communis | dilinolein + linoleate | - |
? | |
| triolein + H2O | - |
Ricinus communis | diolein + oleate | - |
? | |
| tripalmitin + H2O | - |
Ricinus communis | dipalmitin + palmitate | - |
? | |
| triricinolein + H2O | - |
Ricinus communis | diricinolein + ricinolate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, about, SDS-PAGE | Ricinus communis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid lipase | - |
Ricinus communis |
| OBL1 | - |
Ricinus communis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
recombinant enzyme | Ricinus communis |
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