Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in open conformation through complexion by inhibitor Rc-(Rp,Sp)-1,2-dioctylcarbamoyl-glycero-3-O-(4-nitrophenyl) octylphosphonate, sitting drop vapour diffusion method, room temperature, 5 mg/ml protein in10 mM Tris-HCl, pH 8.0, 1% beta-octylpyranoside, equilibration against precipitation solution containing 25% 2-methyl-2,4-pentanediol, 20 mM CaCl2, 100 mM citrate, pH 5.6, several months, X-ray structure determination and analysis at 2.54 A resolution, structure scheme and modelling | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Rc-(Rp,Sp)-1,2-dioctylcarbamoyl-glycero-3-O-(4-nitrophenyl) octylphosphonate | binding site structure, binding arrests the enzyme in the open conformation | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizes the loop containing the catalytic His251 residue, binding site structure | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P26876 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad is formed by Ser82, Asp229, and His251, substrates bind first to Ser82, position of the oxyanion hole and the three pockets accomodating the sn-1, sn-2, and sn-3 fatty acid chains, reaction mechanism, tetrahedral intermediate during acylation step | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
triacylglycerol + H2O | - |
Pseudomonas aeruginosa | diacylglycerol + a carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme structure contains an unusual alpha/beta hydrolase fold lacking two beta-strands and one helix | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Pseudomonas aeruginosa |
More | the enzyme belongs to the family 1.1 of bacterial lipases | Pseudomonas aeruginosa |