Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26500 | - |
isozyme LIP II, gel filtration | Fagopyrum esculentum |
28400 | - |
1 * 28400, isozyme LIP II, SDS-PAGE, 1 * 150000, isozyme LIP I, SDS-PAGE | Fagopyrum esculentum |
150000 | - |
1 * 28400, isozyme LIP II, SDS-PAGE, 1 * 150000, isozyme LIP I, SDS-PAGE | Fagopyrum esculentum |
171000 | - |
isozyme LIP I, gel filtration | Fagopyrum esculentum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fagopyrum esculentum | - |
var. Kitawasesoba, two isozymes LIP I and LIP II | - |
Purification (Comment) | Organism |
---|---|
native isozymes LIP I and LIP II 60fold and 143fold from seed to near homogeneity by ion exchange chromatography and gel filtration, respectively | Fagopyrum esculentum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | high activity | Fagopyrum esculentum | - |
endosperm | low activity compare to the embryo | Fagopyrum esculentum | - |
testis | very low activity | Fagopyrum esculentum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
purified isozyme LIP I, substrate triolein, 30°C | Fagopyrum esculentum |
0.36 | - |
purified isozyme LIP I, substrate 4-nitrophenyl laurate, 22°C | Fagopyrum esculentum |
0.73 | - |
purified isozyme LIP II, substrate triolein, 30°C | Fagopyrum esculentum |
0.86 | - |
purified isozyme LIP II, substrate 4-nitrophenyl laurate, 22°C | Fagopyrum esculentum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Fagopyrum esculentum | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Fagopyrum esculentum | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Fagopyrum esculentum | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl laurate + H2O | best substrate | Fagopyrum esculentum | 4-nitrophenol + laurate | - |
? | |
olein + H2O | low activity with isozyme LIP II, residual activity with isozyme LIP I | Fagopyrum esculentum | glycerol + oleate | - |
? | |
palmitin + H2O | low activity with isozyme LIP II, residual activity with isozyme LIP I | Fagopyrum esculentum | glycerol + palmitate | - |
? | |
triolein + H2O | good substrate | Fagopyrum esculentum | diolein + oleate | - |
? | |
tripalmitin + H2O | low activity with isozyme LIP II, residual activity with isozyme LIP I | Fagopyrum esculentum | dipalmitin + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28400, isozyme LIP II, SDS-PAGE, 1 * 150000, isozyme LIP I, SDS-PAGE | Fagopyrum esculentum |
Synonyms | Comment | Organism |
---|---|---|
LIP | - |
Fagopyrum esculentum |
lipase | - |
Fagopyrum esculentum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
isozyme LIP I, and isozyme LIP I with substrate triolein | Fagopyrum esculentum |
40 | - |
isozyme LIP I with substrate 4-nitrophenyl laurate | Fagopyrum esculentum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 70 | isozymes LIP I amd II, activity is dependent on substrate, fully stable at 10°C and 45% remaining activity at 70°C with substrate triolein after 4 h | Fagopyrum esculentum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
isozyme LIP I with substrate triolein | Fagopyrum esculentum |
5 | - |
isozyme LIP I with substrate 4-nitrophenyl laurate | Fagopyrum esculentum |
6 | - |
isozyme LIP II | Fagopyrum esculentum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | isozymes LIP I and II with substrate 4-nitrophenyl laurate | Fagopyrum esculentum |
3 | 7 | isozymes LIP I and II with substrate triolein | Fagopyrum esculentum |