Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli at 27°C | Pseudomonas fragi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | binding involves residues D217 and D262 | Pseudomonas fragi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fragi | Q9EV86 | gene lips | - |
Pseudomonas fragi IFO 3458 | Q9EV86 | gene lips | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by metal-chelating affinity chromatography, the His-tag is cleaved off by Tev protease | Pseudomonas fragi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, the enzyme prefers triacylglycerides with short fatty acid chains | Pseudomonas fragi | ? | - |
? | |
additional information | substrate specificity, the enzyme prefers triacylglycerides with short fatty acid chains | Pseudomonas fragi IFO 3458 | ? | - |
? | |
tributyrin + H2O | preferred substrate | Pseudomonas fragi | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | preferred substrate | Pseudomonas fragi IFO 3458 | dibutyrin + butyrate | - |
? | |
tricaprylin + H2O | - |
Pseudomonas fragi | dicaprylin + caprylate | - |
? | |
trilaurin + H2O | - |
Pseudomonas fragi | dilaurin + laurate | - |
? | |
trilaurin + H2O | - |
Pseudomonas fragi IFO 3458 | dilaurin + laurate | - |
? | |
trimyristin + H2O | - |
Pseudomonas fragi | dimyristin + myristate | - |
? | |
triolein + H2O | low activity | Pseudomonas fragi | diolein + oleate | - |
? | |
triolein + H2O | low activity | Pseudomonas fragi IFO 3458 | diolein + oleate | - |
? | |
tripalmitin + H2O | - |
Pseudomonas fragi | dipalmitin + palmitate | - |
? | |
tripalmitin + H2O | - |
Pseudomonas fragi IFO 3458 | dipalmitin + palmitate | - |
? | |
tristearin + H2O | low activity | Pseudomonas fragi | distearin + stearate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure modelling and analysis, overview | Pseudomonas fragi |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Pseudomonas fragi |
PFL | - |
Pseudomonas fragi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
29 | - |
- |
Pseudomonas fragi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | 59% of maximal activity at 10°C, 15% at 50°C | Pseudomonas fragi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
60% remaining activity of the purified recombinant enzyme | Pseudomonas fragi |
27 | - |
half-life of the purified recombinant enzyme is about 5 h | Pseudomonas fragi |
50 | - |
nearly complete loss of activity within a few min of the purified recombinant enzyme | Pseudomonas fragi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas fragi |