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Literature summary for 3.1.1.3 extracted from

  • Castro-Ochoa, L.D.; Rodriguez-Gomez, C.; Valerio-Alfaro, G.; Oliart Ros, R.
    Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11 (2005), Enzyme Microb. Technol., 37, 648-654.
No PubMed abstract available
Search for more literature for 3.1.1.3

Inhibitors

Inhibitors Comment Organism Structure
butanol 70%, 30°C, complete immediate inactivation Geobacillus thermoleovorans
Hg+
-
 Geobacillus thermoleovorans
K+
-
 Geobacillus thermoleovorans
Li+
-
 Geobacillus thermoleovorans
Mg2+
-
 Geobacillus thermoleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Geobacillus thermoleovorans
additional information no effect by Ba2+ and Na+ Geobacillus thermoleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
triolein + H2O Geobacillus thermoleovorans olive oil diolein + oleate
-
 ?
triolein + H2O Geobacillus thermoleovorans CCR11 olive oil diolein + oleate
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol 70%, 30°C, completely stable for 1 h, loss of 18% activity after 2h Geobacillus thermoleovorans
Acetone 70%, 30°C, completely stable for 1 h, loss of 60% activity after 2h Geobacillus thermoleovorans
Butanol 70%, 30°C, complete immediate inactivation Geobacillus thermoleovorans
Ethanol 70%, 30°C, completely stable for 1 h, loss of 30% activity after 2h Geobacillus thermoleovorans
Methanol 70%, 30°C, loss of 35% activity after 2h Geobacillus thermoleovorans

Organism

Organism UniProt Comment Textmining
Geobacillus thermoleovorans
-
 isolated from a hot spring in Mexico
-
Geobacillus thermoleovorans CCR11
-
 isolated from a hot spring in Mexico
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from cell-free supernatant by isoelectric focusing, PAGE, and ultrafiltration Geobacillus thermoleovorans

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimal growth at pH 6.5, 55°C in presence of 1% gum arabic, 2.5% olive oil and 0.1% w/v CaCl2 Geobacillus thermoleovorans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O
-
 Geobacillus thermoleovorans 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O
-
 Geobacillus thermoleovorans CCR11 4-nitrophenol + laurate
-
 ?
triolein + H2O olive oil Geobacillus thermoleovorans diolein + oleate
-
 ?
triolein + H2O olive oil Geobacillus thermoleovorans CCR11 diolein + oleate
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Geobacillus thermoleovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Geobacillus thermoleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 6.5, 1 h, completely stable, 26 h, over 80% remaining activity Geobacillus thermoleovorans
50 60 pH 6.5, 1 h, 75% remaining activity Geobacillus thermoleovorans
70
-
 pH 6.5, 1 h, nearly complete loss of activity Geobacillus thermoleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
 Geobacillus thermoleovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 purified enzyme, stable Geobacillus thermoleovorans

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus thermoleovorans isoelectric focusing
-
 6