Cloned (Comment) | Organism |
---|---|
expression of cutinase in Escherichia coli strain BL21(DE3) periplasmic space | Fusarium solani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | P22088 | - |
- |
Fusarium solani | - |
- |
- |
Rhizomucor miehei | - |
- |
- |
Thermomyces lanuginosus | O59952 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant cutinase partially from Escherichia coli strain BL21(DE3) periplasmic space by cation exchange chromatography | Fusarium solani |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | activity required deprotonation of the catalytic His residue | Rhizomucor miehei | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | activity required deprotonation of the catalytic His residue | Burkholderia cepacia | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | activity required deprotonation of the catalytic His residue | Thermomyces lanuginosus | |
triacylglycerol + H2O = diacylglycerol + a carboxylate | activity required deprotonation of the catalytic His188 residue, model of electrostatics in the active site, the active site is essentially covered with lipod surface during catalysis | Fusarium solani |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
pH 4.0, partially purified recombinant enzyme | Fusarium solani |
12.4 | - |
pH 6.0, partially purified recombinant enzyme | Fusarium solani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
triolein + H2O | - |
Fusarium solani | diolein + oleate | - |
? | |
triolein + H2O | - |
Rhizomucor miehei | diolein + oleate | - |
? | |
triolein + H2O | - |
Burkholderia cepacia | diolein + oleate | - |
? | |
triolein + H2O | - |
Thermomyces lanuginosus | diolein + oleate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Fusarium solani |
lipase | - |
Rhizomucor miehei |
lipase | - |
Burkholderia cepacia |
lipase | - |
Thermomyces lanuginosus |
pisi cutinase | - |
Fusarium solani |
triglyceride lipase | - |
Fusarium solani |
triglyceride lipase | - |
Rhizomucor miehei |
triglyceride lipase | - |
Burkholderia cepacia |
triglyceride lipase | - |
Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Fusarium solani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
broad optimum, influence of pH on catalytic mechanism, overview | Thermomyces lanuginosus |
8 | - |
influence of pH on catalytic mechanism, overview | Rhizomucor miehei |
8 | - |
influence of pH on catalytic mechanism, overview | Burkholderia cepacia |
8.5 | - |
influence of pH on catalytic mechanism, pH-activity profile, overview | Fusarium solani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10.5 | pH profile, the enzyme is active under acidic conditions | Fusarium solani |
4 | 10.5 | pH profile, the enzyme is active under acidic conditions and shows 22.4% of maximal activity at pH 4.0 | Rhizomucor miehei |
4 | 10.5 | pH profile, the enzyme is active under acidic conditions and shows 25.3% of maximal activity at pH 4.0 | Burkholderia cepacia |
4 | 10.5 | pH profile, the enzyme is active under acidic conditions and shows 70.6% of maximal activity at pH 4.0 | Thermomyces lanuginosus |