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Literature summary for 3.1.1.3 extracted from

  • Shabtai, Y.; Daya-Mishne, N.
    Production, purification, and properties of a lipase from a bacterium (Pseudomonas aeruginosa YS-7) capable of growing in water-restricted environments (1992), Appl. Environ. Microbiol., 58, 174-180.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
Dimethyl formamide concentrated solution increases the enzyme activity 2fold, maximal activity at a water content of 0.5-1% w/w Pseudomonas aeruginosa
dimethyl sulfoxide concentrated solution increases the enzyme activity 5fold, maximal activity at a water content of 0.5-1% w/w Pseudomonas aeruginosa
additional information alcohols and glycols up to 20% activate the enzyme, high concentrations inhibit, the extracellular enzyme is induced by triolein, soybean oil, Tween 20, Tween 80, and especially by free fatty acids, e.g. oleic acid or lauric acid more than 25fold Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile
-
 Pseudomonas aeruginosa
additional information alcohols and glycols up to 20% activates the enzyme, high concentrations inhibit Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion of the enzyme to culture medium Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, about, SDS-PAGE Pseudomonas aeruginosa

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl formamide the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethyl sulfoxide Pseudomonas aeruginosa
dimethyl sulfoxide the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethyl sulfoxide Pseudomonas aeruginosa
additional information the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethylsulfoxide Pseudomonas aeruginosa
Pyridine the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethylsulfoxide Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa YS-7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from culture supernatant by acetone precipitation and hydrophobic interaction chromatography Pseudomonas aeruginosa

Source Tissue

Source Tissue Comment Organism Textmining
additional information culture conditions evaluation, overview Pseudomonas aeruginosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.22
-
 purified enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa YS-7 4-nitrophenol + laurate
-
 ?
soybean oil + H2O concentrated soybean oil, high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa ?
-
 ?
soybean oil + H2O concentrated soybean oil, high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa YS-7 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, about, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
lipase
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 55 purified enzyme, stable for over 30 days in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethyl sulfoxide Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas aeruginosa