Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by EDTA | Bacillus sp. (in: Bacteria) |
Application | Comment | Organism |
---|---|---|
detergent | the enzyme is usable with laundry detergents | Bacillus sp. (in: Bacteria) |
General Stability | Organism |
---|---|
Ca2+ stabilizes the enzyme at concentration of 1-10 mM with decreasing effect at higher concentration, 100% activity at 1 mM, 4% activity at 10 mM | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Bacillus sp. (in: Bacteria) | |
ammonium persulfate | - |
Bacillus sp. (in: Bacteria) | |
ascorbic acid | - |
Bacillus sp. (in: Bacteria) | |
Co2+ | - |
Bacillus sp. (in: Bacteria) | |
Cs+ | - |
Bacillus sp. (in: Bacteria) | |
Cu2+ | - |
Bacillus sp. (in: Bacteria) | |
deoxycholate | - |
Bacillus sp. (in: Bacteria) | |
K+ | - |
Bacillus sp. (in: Bacteria) | |
Mn2+ | - |
Bacillus sp. (in: Bacteria) | |
additional information | not affected by EDTA | Bacillus sp. (in: Bacteria) | |
Ni2+ | slight inhibition | Bacillus sp. (in: Bacteria) | |
potassium iodide | - |
Bacillus sp. (in: Bacteria) | |
Sn2+ | - |
Bacillus sp. (in: Bacteria) | |
Tween-20 | - |
Bacillus sp. (in: Bacteria) | |
Zn2+ | - |
Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
4-nitrophenyl butyrate | pH 8.0, 50°C | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bacillus sp. (in: Bacteria) | |
additional information | the enzyme is no metalloenzyme, not affected by citrate, Ba2+, K+, and Mg2+ | Bacillus sp. (in: Bacteria) | |
Na+ | slight activation | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
thermostable alkaline lipase | - |
Bacillus sp. (in: Bacteria) RSJ-1 | - |
thermostable alkaline lipase | - |
Oxidation Stability | Organism |
---|---|
enzyme retains 93% activity at 0.1% H2O2, 74% activity at 0.2% H2O2, and 47% activity at 1% H2O2 | Bacillus sp. (in: Bacteria) |
Purification (Comment) | Organism |
---|---|
to homogeneity from culture supernatant, 201fold | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
428.1 | - |
purified enzyme, substrate 4-nitrophenyl butyrate | Bacillus sp. (in: Bacteria) |
Storage Stability | Organism |
---|---|
enzyme is stable in nonionic detergents such as Triton X-100, Tween-80, and Tween-20, but is less stable in presence of anionic detergents e.g. deoxycholate | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Bacillus sp. (in: Bacteria) RSJ-1 | 4-nitrophenol + butyrate | - |
? | |
triolein + H2O | - |
Bacillus sp. (in: Bacteria) | diolein + oleate | - |
? | |
triolein + H2O | - |
Bacillus sp. (in: Bacteria) RSJ-1 | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable for 60 min, after 120 min 90% remaining activity | Bacillus sp. (in: Bacteria) |
55 | - |
half-life 240 min | Bacillus sp. (in: Bacteria) |
60 | - |
half-life 150 min | Bacillus sp. (in: Bacteria) |
65 | - |
half-life 90 min | Bacillus sp. (in: Bacteria) |
70 | - |
half-life 45 min | Bacillus sp. (in: Bacteria) |
75 | - |
half-life 30 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 12 | 12% of maximal activity at pH 5.0, 18% of maximal activity at pH 12.0 | Bacillus sp. (in: Bacteria) |