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Literature summary for 3.1.1.3 extracted from

  • Nawani, N.; Kaur, J.
    Purification, characterization and thermostability of lipase from a thermophilic Bacillus sp. J33 (2000), Mol. Cell. Biochem., 206, 91-96.
    View publication on PubMed
Search for more literature for 3.1.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 4-nitrophenyl laurate pH 8.0, 60°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 1 * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)
46500
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
triolein + H2O Bacillus sp. (in: Bacteria)
-
 diolein + oleic acid
-
 ?
triolein + H2O Bacillus sp. (in: Bacteria) J33
-
 diolein + oleic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) J33
-
-
-

Purification (Commentary)

Purification (Comment) Organism
175fold Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1046
-
 purified enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O
-
 Bacillus sp. (in: Bacteria) J33 4-nitrophenol + laurate
-
 ?
additional information fatty acid substrate specificity is broad with little preference for C12 and C4 Bacillus sp. (in: Bacteria) ?
-
 ?
additional information fatty acid substrate specificity is broad with little preference for C12 and C4 Bacillus sp. (in: Bacteria) J33 ?
-
 ?
tributyrin + H2O
-
 Bacillus sp. (in: Bacteria) dibutyrin + butyric acid
-
 ?
tributyrin + H2O
-
 Bacillus sp. (in: Bacteria) J33 dibutyrin + butyric acid
-
 ?
tricaprin + H2O
-
 Bacillus sp. (in: Bacteria) dicaprin + capric acid
-
 ?
tricaproin + H2O
-
 Bacillus sp. (in: Bacteria) dicaproin + caproic acid
-
 ?
tricaproin + H2O
-
 Bacillus sp. (in: Bacteria) J33 dicaproin + caproic acid
-
 ?
tricaprylin + H2O
-
 Bacillus sp. (in: Bacteria) dicaprylin + caprylic acid
-
 ?
trilaurin + H2O
-
 Bacillus sp. (in: Bacteria) dilaurin + lauric acid
-
 ?
triolein + H2O
-
 Bacillus sp. (in: Bacteria) diolein + oleic acid
-
 ?
triolein + H2O
-
 Bacillus sp. (in: Bacteria) J33 diolein + oleic acid
-
 ?
tripalmitin + H2O
-
 Bacillus sp. (in: Bacteria) dipalmitin + palmitic acid
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
lipase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 12 h stable, immobilized enzyme, thermal stabilization by 2.5 M glycerol, 2.5 M sorbitol, and 2.5 M ethylene glycol Bacillus sp. (in: Bacteria)
70
-
 stable, no deactivation after 150 min in presence of additives Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus sp. (in: Bacteria)